RATES OF INHIBITIOX OF PURE ENZYMES 



0.2 



549 



iLO 



Inhibition 



0.8 



— 0.6 



0.4 



02 



MIN 



Fig. 12-11. Reaction of lactate dehydrogenase sulfhydryl groups with j.)-chloromer- 



curibenzoate (p-CMB) measured spectrophotonietrically and the time course of the 



inliibition. For the sulfhydryl reaction, (p-CMB) = 0.033 mJ/ and (LDH) = 0.01 ml/; 



for the inhibition, (jj-CMB) = 0.1 mM. (From Xeilands. 1954.) 



100 



60- 



40 



20- 



% 

 Inh., 



TIME- 



30 



60 



90 



MIN 



120 



Fig. 12-12. Inhibition of succinoxidase by several sulfhydryl 



reagents. GSSG = oxidized glutathione, PAPAO = p-aminophen- 



yjarsine oxide, ^^-CMB ^ y»-chloromercuribenzoate, and ISBZ = 



iodosobenzoatc. Temperature = 37°. (From Slater. 1949.) 



