668 



14. EFFECTS OF pH ON ENZYME INHIBITION 



K, ES ^ E + P 



E 



K, EHI 



(14-46) 



I -f H =± HI 



Fig. 14-6. Effect of the pH on the inhibition of 



papain by carbobenzoxy-L-ghitamate (20 miH). (From 



Stockell and Smith, 1957.) 



the fractional inhibition is given by: 



(14-47) 



which is identical to the usual expression for competitive inhibition (Eq. 

 3-13) except for the replacement of the apparent inhibitor constant with 

 f/n'K'. If the inhibitor is di- or tribasic, the proper/" of/'" functions may 

 be used. 



