VARIATION OF ENZYME INHIBITION IWTH pH 



671 



(c) HE is active form of enzyme: I combines with HE noncompetitively. 



HE +P 



K, HES A'. 



Ka ^ ^ 



E — HE HESI 



>^ ^ 



A'i HEI A-, 



(14-59) 



It should be noted that completely noncompetitive inhibition is assmned; 

 i.e., there is no effect of S on the binding of I or of I on the binding of S. 



(14-60) 



(I) 



(I) + K, 



1 + 



K„ 



K, 



(14-61) 



(H) (S) + K, 



(d) E is active form of enzyme: I combines with E noncompetitively. 



E + P 



K, ES A- 

 A'. ^ ^' 



HE ^ E ESI 



A. EI A-. 



(14-62) 



Vi = -p 



F,„(S) 



(I) + K, 



1 + 



(H) K, 



(14-63) 



(14-64) 



Ka (S) + K, 



(e) HE is active form of enzyme: I combines with E. 



A, A-„ A-, 



EI ^ E ;^ HE ^ HES ^ HE + P 



Vi 



VJS) 



(14-65) 

 (14-66) 



(14-67) 



(I) +K 



