PANTOTHENIC ACID 643 



significantly increased, particularly for Lactobacillus casei and Lacto- 

 bacillus fermenti. After complete inhibition of growth of these organisms 

 was attained, further increases in the concentration of the analogue stimu- 

 lated growth of the organisms. Thus, at high concentrations of pantothenic 

 acid, complete inhibition of growth is never attained, even though the 

 analogue alone is incapable of stimulating growth of the organisms. 



w-Methylpantothenic acid is effective against streptococci both in vitro 

 and in vivo. Mice were protected from an 80 per cent fatal infection of 



Table 33. Comparison of Activity of u-Methylpantoyl Derivatives with Pantoyltaurine.™ 



Organism 



Leuconostoc citrovorum 8082° 

 Lactobacillus fermentatus 4006 

 Lactobacilhis pentoaceticus 367 

 Lactobacillus brevis 8257 

 Leuconostoc citrovorum 797 

 Leuconostoc citrovorum 7013 

 Streptococcus faecalis R 8043 

 Lactobacillus casei 7469 

 Lactobacillus helveticus 335 

 Lactobacillus helveticus 6345 

 Lactobacillus lycopersici 4005 

 Leuconostoc dextranicum 8358 

 Leuconostoc dextranicum 8086 

 Leuconostoc mesenteroides 9135 

 Leuconostoc mesenteroides 8293 

 Lactobacillus fermenti 36-9338 

 Lactobacillus gayoni 8289 

 Leuconostoc dextranicum 8359 

 Leuconostoc mesenteroides P-60 (8042) 

 Lactobacillus pentosus 124-2 

 Lactobacillus arabinosus 17-5 (8014) 

 Lactobacillus brassicae 8041 

 Lactobacillus mannitopoeus 



° American Type Culture Collection number. 



b Stimulation of growth obtained at concentrations just below inhibitory levels. 

 c Half-maximum inhibition; complete inhibition of growth not obtained at index of 280,000. 

 d Stimulation of growth, no inhibition obtained. 



• Stimulation of growth obtained at concentrations of analogue higher than that just necessary for 

 maximum inhibition. Effect enhanced by higher concentrations of pantothenic acid. 



a /^-hemolytic streptococcus (Group A, type 23, No. 1072) when the 

 analogue was administered in the diet for four days prior to infection at 

 a concentration 200 times that of pantothenic acid. 



Production of a pantothenic acid deficiency in weanling mice has been 

 accomplished by supplementation of the diet with w-methylpantothenic 

 acid. 90 On a pantothenic acid-deficient diet, the survival time decreased 

 from 8 to 9 weeks to 5, 4, 2.5 and 1.5 weeks by supplementation with 0.06, 

 0.2, 0.5 and 1.5 per cent, respectively, of the analogue in the diet. With 

 diets containing 2 and 6 mg per cent of pantothenic acid and a 100-fold 

 excess of the analogue, the survival time was about the same as on the 



