HYPOPHYSEAL MORPHOLOGY 



191 



Fig. 3.15 (upper Icjt). Section 

 aldehyde-fuchsin (AF). The cells 

 granules to be concentrated at the 



1mi,. :;.1:] i '-/'/" - "<//'/ '• >< '-'idii of the mt 

 pans aiUciiur showiug, iulliclf-.-liiuulating lior- 

 mone cells stained by periodic acid-Schiff 

 (PAS). The cells are ovoid in shape and con- 

 tain clumps of densely stained granules. PAS, 

 X 720. 



Fig. 3.14 (lower left). Section of the rat 

 pars anterior showing luteinizing hormone 

 cells stained by periodic acid-Schiff (PAS). 

 ■M The cells have rounded con tours and the 



la basophil granules are more unil'orinly dis- 



Wa perscd throughout the cytoplasiu than in the 



^ other two basophil types. One cell near the 



center of the field shows a prominent nega- 

 tive image of the Golgi body. The cytoplasm 

 enclosed by the Golgi body has a higher 

 ■* ' granule content that the peripheral cyto- 



plasm. PAS, X 720. 

 of the rat pars anterior showing thyrotrophs stained by 

 have angular contours and there is a tendency for the 

 periphery. AF, X 720. 



created number of basophils is due to an in- 

 crease in conijiact cells which are oval or 

 round. 



The McManus (1946) PAS reaction for 

 the demonstration of glycoproteins in histo- 

 logic sections initiated researches based on 

 the idea that this reaction might demon- 

 strate the hormone content of basophil cells. 

 Herlant (1949, 1951) and Pearse (1949, 

 1951 ) ascribed the reaction given by baso- 

 phil granules to the presence of gonado- 

 trophin. Catchpole (1949) found in castra- 

 tion cells and in certain basophil cells of 

 normal animals glycoproteins which could 

 have been gonadotrophins. He looked for 

 but did not find glycoprotein with the solu- 

 bility of thyrotrophin in thyroidectomy 

 cells. He considered that the glycoprotein 



staining did not parallel the staining with 

 aniline blue. 



Purves and Griesbach (1951a) set out to 

 test the hypothesis that the PAS reaction 

 could. be used to demonstrate the hormones 

 of basophil cells by examining rat hypophy- 

 ses in which thyrotrophin or gonadotrophin 

 or both were absent. Such hypophyses were 

 obtained by treatment of intact rats with 

 thyroxine or estrogen or both thyroxine and 

 estrogen. In hypophyses that contained only 

 thyrotrophin the glycoprotein reaction and 

 the aniline blue-stained granules were con- 

 fined to cells that were polyhedral in shai:)e 

 and were distributed throughout the interior 

 of the pars anterior. In hypophyses contain- 

 ing only gonadotrophins the glycoprotein 

 reaction and the aniline blue-stained grnn- 



