208 



HYPOPHYSIS AND GONADOTROPHIC HORMONES 



f^'^ 



Fig. 3.19 (upper left). Section of the human pars distalis showing weak staining of the 

 granules of purple ;3-cells by aldehyde-fuchsin after fixation in Helly's fluid. Much of the 

 density of the purple /3-cells in the photomicrograph is due to the reddish tint given to them 

 by the azan counterstain. A single blue jS-cell (B) is strongly stained by the aldehyde-fuchsin 

 (AF). In the section it appeared an indigo color from superimposition of a blue color from the 

 aniline blue of the counterstain. AF, azan, X 900. 



Fig. 3.20 (upper right). Section of the human pars distalis showing 7-cells. The cells here 

 show elongated forms. The granulation was stained a weak purplish shade. AF, azan, X 900. 



Fig. 3.21 (lower left). Section of the human pars distalis showing 7-cells stained by periodic 

 acid-Schiff (PAS). The conspicuous PAS +ve droplets which are unusuallj^ numerous in this 

 field are not peculiar to the 7-cell since they may be found in other cell types, both basophil 

 and acidophil. The faint grey granulation distributed throughout the cytoplasm is considered 

 to be the specific 7-granulation. PAS, X 900. 



Fig. 3.22 (lower right). Section of the human pars distalis fixed in Helly's fluid and stained 

 with aldehyde-fuchsin (AF), counterstained with azan. Acidophils, blue /3-cells and purple 

 /3-cells which were deeply stained in different colors in the section, appear black and cannot 

 be clearly distinguished from one another in the photomicrograph. The numerous large 

 rounded pale cells with grey granulation are 5-cells. The granules were stained a clear blue in 

 the original. AF, azan, X 900. 



Alei;iii blue comprise the blue /i- and 8-cclls. 

 Herlant (1956b) obtained the same distri- 

 bution of the aldehyde-fuchsin stain in sec- 

 tions oxidized with permanganate. It is to 

 be noted that the effect of oxidation is to 

 inhibit the staining of purple /?-cells by 

 aldehyde-fuchsin and to render the 8-cells 



stainal)lc; the hhic /i-cells are stained with 

 or without oxidation. The effects of jier- 

 manganate oxidation on the aldehyde-fuch- 

 sin staining of the human pars distalis are 

 the same as those observed in the pars an- 

 terior and pars intermedia of the rat (Halmi 

 and Davies, 1953). The staining of the pars 



