404 



PHYSIOLOGY OF GONADS 



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Fig. 6.27. R:it xcniinl |.in~i,ii( ikhihiI mil. I ,li . 1 1 uiiiuicrograpli X 8500; LuftV ])prman- 

 ganate fixative. Aliciuvilli lxIlikI a^ i)iul()n^atioii.-. ol the cytoplasm into the lumen; a major 

 part of the cytoplasm is a labyrinth of ergastoplasmic sacs with scattered mitochondria ; nu- 

 clei are basal ; half-way between nucleus and cell apex is a zone of small vesicles and canals, 

 the Golgi complex (From J. C. Harkin, un])u})lishe(l.) 



stained area in the ai)ical cytoi)lasin was 

 not clear. Ki.xoii and Whitfield 11959) re- 

 ported high concentrations of zinc in the 

 apical cytoplasm, nucleoli, and stroma in 

 fixed tissues stained with dithizone. Tn the 

 apical cytoplasm, the zinc is conccnti atcd 

 at the tip of the cells in a "luminal l)order 

 organelle" which is osmiophilic (distinct 

 from the (Jo].o;i apparatus), argent()i)hilic, 



and basophilic. Nucleoli and subepithelial 

 sti'oma are basophilic. 



Castration results in a typical pattern 

 of involution in the epithelial cells: size is 

 !•(■( bleed, nuclei become small and jwknotic, 

 and changes occur in the density of the cy- 

 toplasm (Korenchevsky and Dennison, 

 1935; Price, Mann and Lutwak-Mann, 

 1955). The zinc content of the gland (dor- 



