406 



PHYSIOLOGY OF GONADS 



■:^lVi4 



l'"i(..G.2U. Rat \(;iilial lUo.-Uilu, iiuinial iiiak;. I'^lcctionnu' 

 acid fixative with sucrose. Supranuclear region of epithelia 

 plasmic sacs. (From J. C. Harkin, unpublished.) 



.-laph Is.iiOO; Paladps osmic 

 •ell sliowing laniellatetl ergasto- 



in the lateral lolje. Tiiere is no diffuse stain- 

 ing of the apical cytoplasm. Nucleoli arc 

 intensely zinc-positive after fixation and 

 staining with dithizone; nucleoli, apical cy- 

 toplasm and stroma are basophilic fRixon 

 and Whitfield, 1959). The stroma is also 

 strongly alkaline phosphatase - positive 

 (Bern, 1949a). 



Epithelial cells respond to castration l»y 

 reduction in cell and nuclear size, and loss 

 of granulation in the cytoplasm (Koren- 

 chevsky and Dennison, 1935). Brandes and 

 Portela (1960a) observed in electron micro- 

 graphs the V)eginning of collapse of the 



cisternae of the endoi)lasmic reticulum, re- 

 duction in RNA-rich particles, and changes 

 in mitochondria. Histochemical studies 

 ( Iicni and Levy, 1952) indicate that distri- 

 bution of alkaline phosphatase activity re- 

 mains unchanged. Fructose content is re- 

 duced in the gland after castration (Price, 

 Mann and Lut\vak-]\Iann, 1955). 



CocKjulating gland {anterior prostdte) . In 

 normal males, the ei)ithelium is columnar 

 and rests on a well marked basement mem- 

 brane; nuclei stain heavily and homogene- 

 ously and ai'e situated midway between the 

 basement meml)i';iiie and lumen. The cvto- 



