ACCESSORY MAMMALIAN REPRODTTCTIVE GLANDS 



4i; 



cy 



/Jf-!^ 



pc 



N.pc 



:y 



. .... ^ 



cy 



\ 



m 



..^cy 



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^m 



V*' 



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cy 





IS 



Fig. 6.37. Mouse coagulating gland, normal male. Electronmicrograph X 39,000. Caulfield's 

 modification of Palade's osmic acid fixative. Basal portion of an epithelial cell; insert, de- 

 tails of basement membrane region: bm, basement membrane: ci, dilated cisternae: cm, 

 plasma or cell membrane; cy, cytoplasmic matrix; ?n, mitochondrion: /;, nucleus. (From D. 

 Brandes, unpublished.) 



centrations of RNA basally and apically in 

 the epithelial cells. Strong protein reactions 

 are present in luminal secretion and apical 

 regions of the cells. 



The response to gonadectomy (Brandes 

 and Bourne, 1954) includes reduction of al- 

 kaline and acid phosphatase activity within 

 4 days, PAS reactions by 8 days, and slight 



fragmentation and loss of density of the 

 Golgi apparatus by 12 to 14 days. Changes 

 are more marked after longer periods of 

 castration (Table 6.8), although Bern 

 (1951) observed retention of stromal alka- 

 line phosphatase for long periods. RNA 

 concentrations in the cell (Bern, Alfert and 

 Blair, 1957) are greatly decreased but some 



