JOHN W. CHURCHMAN 



25 



TABLE I 



Gram.Positive Organisms 



Gram-Negative Organisms 



1. Killed bacteria not digested by trypsin 

 or pepsin (Kantorowiecz) * 



2. Only a few are digested by gastric juice 

 (Burgers) t 



3. Resistant to alkalies; not dissolved by i 

 per cent KOH (Kruse,t Smith§) 



4. Lipoids resistant to fat solvents (Jobling 

 and Petersen) 1 1 



5. Degree of dispersion of nucleoproteins 

 high (Hottinger)^ 



6. Sensitive to weak electrolytes (dyes) 

 Smith, § Burke**) 



7. Optimum growth at relatively high pH 

 (Burke)** 



8. Resistant to non-electrolytes (Smith) § 

 Q. Limited iso-electric staining range cen- 

 tered about pH 2-3 (Stearns and 

 Stearns) ft 



10. For the most part (about 10 per cent 

 exceptions) very susceptible to bacterio- 

 static effect of triphenyl-methane dyes 

 (Churchman) ft 



11. May produce spores 



12. In the living state, more readily per- 

 meated by dyes (Benians)§§ 



13. May be acid fast 



14. More susceptible to quinine and hydro- 

 cuprein derivatives (Traube);^|| 



15. More susceptible to iodine (Traube)ll|| 



16. More susceptible to mesohematin, mag- 

 nesium, and manganese derivatives of 

 mesoporphyrin (Kammerer)TfTf 



17. More readily adsorbed because of surface 

 lipins (Eisenberg)*** 



18. Adsorb halogens less strongly (Breinl) fff 



19. Unaffected by toluol provided no 

 emulsoid be added (Benians)ttt 



* Munchen. med. Wchnschr., 56, 897. 1909. 



\ Zlschr.f. Hyg. u. Infeklionshrankh., 70, 119. 1911. 



X MUnchen. med. Wchnschr. 57, 685. 1910. 



^Am. J. Hyg., 2, 607. 1922. 



II /. Ex per. Med., 20. 456. 1914. 



H Cenlralbl.f. Bakkriol., 76, 367. 1916. 



**/. Bad., 7, 159. 1922. 



tt Ibid., g, 463, 479, 491. 1925; 10, 13. 1925. 



ttJ. Exper. Med., 16, 221. 1912. 



§§ /. Palh. &* Bad., 17, 199. 191 2. 



nil Zlschr.f. Immunitdtsforsch. u. exper. Therap., 29, 2861. 



'ill Arch. f. exper. Palh. u. Pharmakol., 88, 247. 1920. 



*** Centralbl. f. BaklerioL, 54, 145. 1912; ibid., 81, 72. 191 



ttt Zlschr.f. Immunildtsforsch. u. exper. Therap., 29, 343. 



XXX Zlschr.f. Chemolherapie, 2, 28. 1913. 



1. Killed bacteria digested by trypsin and 

 pepsin 



2. Majority digested by gastric juice 



3. Dissolved by i per cent KOH 



4. Lipoids less resistant to fat solvents 



5. Degree of dispersion of nucleoproteins 

 low 



6. Resistant to weak electrolytes 



7. Optimum growth at relatively low pH 



8. Less resistant to non-electrolytes 



9. Wider iso-electric staining range cen- 

 tered about pH 5 



ID. For the most part less susceptible to 

 bacteriostatic effect of triphenyl-meth- 

 ane dyes 



11. Never (?) produce spores 



12. Less permeable to dyes, in living state 



13. Never acid fast 



14. Less susceptible to quinine and hydro- 

 cuprein derivatives 



15. Less susceptible to iodine 



16. Less susceptible to these substances 



17. Less readily adsorbed 



18. Adsorb halogens more readily 



19. Killed by toluol 



