1 86 



SURFACE TENSION OF THE MENSTRUUM 



soap solution of proper concentration. After dispersion the toxin is rendered non- 

 toxic by adsorption about the soap molecule. 



TABLE I 

 Effect of Varying the Concentration of the Toxin Using i Per Cent Soap 



Guinea Pig No. 



3 

 4 

 5 

 6 



7 

 8 



9 



Cone, of 



Toxin, L + 



per cc. 



•715 

 •417 

 . 264 

 .185 

 .158 

 •135 

 .119 

 • 105 

 .096 



Dose, L + 



Conc. of 



Soap in Per 



Cent 



Total V^olume 

 Injected, 



7-4 

 8.4 

 9.4 



Result 



Died in 3 days 

 Died in 3 days 

 Died in 3 days 

 Died in 6 days 



Lived 



Lived 



Lived 



Lived 



Lived 



TABLE II 



Effect of Varying the Concentration of the Soap 



Guinea Pig No. 



Cone, of 



Toxin. L-|- 



per cc. 



Dose, L + 



Total Volume 

 Injected, 



Cone, of 



Soap in Per 



Cent 



Result 



2 

 3 

 4 



S 

 6 



7 

 8 



9 



71S 

 715 

 715 

 715 



715 

 715 

 715 

 715 

 715 



1-4 

 1-4 

 1-4 

 1-4 



1-4 

 1-4 

 1-4 

 1-4 

 1-4 



■715 

 1-413 

 2.14 

 2.86 



3-57 

 4.28 

 S.oo 



571 

 6.42 



Died in 3 days 

 Died in II days 

 Died in 28 days 

 Showed paralysis in 

 40 days. Lived 



Lived 



Lived 



Lived 



Lived 



Lived 



TABLE III 

 Effect of Dilution upon a Soap-Toxin Mixture 



In view of the fact that a number of bacterial toxins, bacteria, and filterable 

 viruses' are detoxified by soap, without destruction of their antigenic properties,^ 

 it should be possible to develop this principle to the point where it would be practi- 

 cable to immunize against several infections in the same injection. 



' McKinley, J- C., and Larson, W. P.: Proc. Soc. Exp. Biol, and Med., 24, 297. 1927. 

 ' Larson, W. P., and Eder, H.: Jour. Amer. Med. Assoc, 86, 998. 1926. 



