M. H. SOULE 



257 



To avoid repetition, it may be well to state that in each of these experiments the pro- 

 cedure was essentially the same. Since the protozoa studied do not live in the absence 



TABLE I 



Showing Calculation of Results in Determination of Corrected Real Respiratory 

 Quotient for B. subtUis Grown on Glycerol Agar, 2 Tubes, 19 Days 



F„ = 



Cc. at 0°, 760 mm. 



CO2 in 2 agar tubes 

 COj in water tube 



V 



B-T 



1+0.003665/ 760 



= 1362 . 03 cc. a t 0,° 760 mm. 



= 2.398 

 = 0.674 



241.249 

 Corr. real resp. quot. = = o. S482 



CO2 in 2 agar controls 



CO2 dissolved 

 CO2 gaseous 



Total CO2 

 O2 loss 



284. 419 



Resp. quot. of glycerol 

 Per cent dissolved CO2 

 Tension dissolved CO2 

 Tension gaseous CO2 

 CO2 quotient 



= 0.857 

 = o. 1875 



1.32 mm 

 = 127. 50 mm 

 = o.oio 



241.249 

 284. 419 



* Obtained by multiplying the foregoing culture values by the nitrogen factor -^ = o.g665. 



01.70 



of blood, no attempts were made to determine quotients for these organisms on blood- 

 free media. 



A comparison of the quotients determined by experiment with the theoretical 

 values given in the last line is of special interest. In every case, excepting the diph- 



