496 THE SPIROCHETES 



Plate IX 

 (Pathogenic Treponemas, Magnification, Xi,ooo) 



Fig. 88. — Treponema pallidum, preparation from chancre; Giemsa's stain 



Fig. 89. — Treponema pallidiitn, preparation from chancre; stained with fuchsin after fixation in 

 buffered formalin. 



Fig. 90. — Treponema pallidum in brain from patient dead of general paralysis; silver impregna- 

 tion. 



Fig. 91. — Treponema pallidum in testicular tissue of rabbit inoculated with paretic brain tissue; 

 silver impregnation. 



Fig. 92. — Treponema pallidum, pure culture; darkfield view 



Fig. 93. — Treponema pallidum, pure culture; darkfield view; young culture two weeks old, 

 grown on ascites agar at 37° C. 



Fig. 94. — Treponema pallidum in the testicular tissue of a rabbit inoculated with pure culture; 

 silver impregnation. 



Fig. 95. — Treponema pertenuc, Giemsa's stain. (After Nichols) 



Fig. 96. — Treponema pcrlemic; pure culture; darkfield view 



Fig. 97.- — Treponema cuniculi in spontaneous lesion of rabbit; Giemsa's stain 



Fig. 98. — The same, fi.xed in buffered formalin and stained with fuchsin 



Figs. 99-100. — Treponema cuniculi as seen by darkfield 



Plate X 

 (Treponemas of the Mouth, Magnification, X 1,000) 



Fig. ioi. — Darkfield view of tartar, showing a specimen of Treponema vincenti (above) and one 

 of Treponema buccale (below) ; darkfield view. 



Fig. 102. — Fontana stain of tartar, showing Treponema buccale (above) and Treponema macro- 

 dentium (below). 



Fig. 103. — Fontana stain of tartar, showing Treponema macrodcntium, Treponema buccale, and 

 Treponema microdentimn. 



Fig. 104. — Treponema microdenlium; darkfield view 



Fig. 105. — Treponema microdenlium; stained with gentian violet, after mordanting 



Fig. 106. — Fontana stain of tartar, showing Treponema microdenlium and Treponema skoliodon- 

 tum (?), as well as two specimens of Treponema macrodenlium. 



Figs. 107-8. — Treponema macrodenlium, as seen by darkfield, and in Fontana preparation 



Fig. 109. — Pure culture of Treponema microdenlium; darkfield view 



Fig. iio. — Pure culture of Treponema macrodenlium; darkfield view 



Fig. III. — Pure culture of Treponema mucosum; darkfield view • 



Plate XI 

 (Treponemas of the Genitalia, Magnification, X 1,000) 



— Treponema refringens in smegma; Fontana stain 



Treponema refringens, pure culture; darkfield view 



Fontana stain of smegma, showing several specimens of Treponema caUigyriim 



Pure culture of Treponema calligyrum; darkfield view 

 , — Treponema genitalis in smegma; Fontana stain 



Treponema genii al is in smegma; darkfield view 



Pure culture of Treponema genitalis, gentian-violet stain, after mordanting 



Plate XII 

 (Leptospira Group, Magnification, X 1,000; All Figures Except 119-20 Darkfield Views) 



Fig. 119. — Stimson's preparation (1907), showing Leptospira interrogans in human kidney; 

 silver impregnation; preparation presented by Dr. Stimson. 



Fig. 120. — Leptospira interrogans in the blood in experimental yellow fever (guinea pig); 

 Giemsa's stain. 



