W. H. MANWARING 1003 



freezing point of anaphylactic serum, lymph, and peritoneal fluid,' also changes in electrical 

 conductivity, index of refraction, and specific rotation, and in the osmotic resistance of 

 erythrocytes. Serological methods have been used to test the parallelism, or lack of parallel- 

 ism, between the precipitin content of a serum and its power to produce passive sensitiza- 

 tion,^ also to a study of the topographical distribution of specific foreign proteins in hyper- 

 sensitive animals, and to the specific protein affinity of perfused hypersensitive tissues.^ By 

 an electro-osmotic method. Otto and Shirakawa*) report the separation of specific sensitiz- 

 ing antibody from specific precipitin. 



' Fleisher, M. S., and Mayer, L. L.: J. Immunol., 9, 319. 1924. 



^'Lake, G. C, Osborne, J. B., and Wells, H. G.: /. Infect. Dis., 14, 364. 1914; Weil, R.: op. cit., 

 I, I. 1916. 



3 Manwaring, W. H., Kasama, Y., and Crowe, H. E.: /. Immunol., 2, 511. 1917. 



4 Otto, R., and Shirakawa, T.: Ztschr.f. Hyg. u. Infektionskrankh., 103, 426. 1924. 



