54 MEMOIES OF THE NATIONAL ACADEMY OF SCIENCES. 



solute alcohol and ether, and, after drying over sulphuric acid, weighed 10.30 grams. Dried at 

 110° C, it contained 12.36 per cent of nitrogen. The filtrate from 2 was concentrated to a thick 

 sirup. On cooling, a deposit formed on the bottom of the dish. The sirup was poured oft' and 

 this deposit treated successively with strong alcohol, absolute alcohol, ether, and finally with ab- 

 solute alcohol again. Afterdrying over sulphuric acid, this precipitate, "3," weighed G.S2 grams, 

 and contained, when dried at 110° C, 10.37 per cent of nitrogen. 



The alcoholic liquids obtained by washing preparations 1,2 and 3 were found to have dissolved 

 a large amount of proteid; they were, therefore, united and concentrated on the water bath to 

 small volume, and cooled. The abundant deposit was washed with absolute alcohol, ether, absolute 

 alcohol again, and dried over sulphuric acid. The 14.62 grams thus obtained, "4," when dried at 

 110° C, contained 16.27 per cent of nitrogen. The filtrate from 4 was still further concentrated, 

 and after cooling and adding a large amount of alcohol, gave a precipitate, "5," which, when 

 treated as the others, weighed 4.21 grams and contained 6 per cent of nitrogen. The filtrate from 

 5 was highly concentrated and treated with absolute alcohol. The precipitate thus thrown dowu, 

 "6," after treating with absolute alcohol, ether, absolute alcohol again, and drying over sulphuric 

 acid, weighed 17.78 grams and contained 8.61 per cent of nitrogen. 



To sum up: From the alcoholic extract, by concentrating and .cooling three times, there 

 resulted three preparations, viz: 



1, of 15.5 grams, containing 13.92 per cent of nitrogen. 



2, of 10.30 grams, containing 12.36 per cent of nitrogen. 



3, of 6.82 grams, containing 10.37 per cent of nitrogen. 



From the alcoholic washings of 1, 2, and 3, by concentration and cooling was obtained prepa- 

 ration 



4, of 14.62 grams, containing 16.27 per cent of nitrogen. 



And by further concentration and addition of much strong alcohol, 



5, of 4.21 grams, containing 6 per cent of nitrogen. 



6, of 17.78 grams, containing 8.61 per cent of nitrogen. 



The principal impurity recognized in these preparations was sugar, which was abundant in 5 

 and 6. 



These six preparations were examined separately. 



1 was warmed witli alcohol of 75 per cent, but was only partly soluble, and owing to its slimy 

 nature, could not be filtered. Three volumes of strong alcohol \fere added, making the solvent 

 equivalent to 2 liters of about 88 per cent alcohol. After heating to boiling and cooling, a little 

 Mrbstance separated, which was filtered out, together with tlie undissolved portion. This precipi- 

 tate and residue, " 1 'A," after treatment with absolute alcohol, ether, etc., as before described, 

 weighed 3.68 grams and contained 14.57 per cent of nitrogen. 



The filtrate from 1 A, concentrated to one-third and cooled, deposited but little substance. 

 Addition of an equal volume of water produced a precipitate. The liquid was poured off and the 

 deposit treated with a large amount of absolute alcohol. The residue, " 1 B," after drying, weighed 

 3.05 grams and contained 15.39 per cent of nitrogen. Water was added to the filtrate from 1 B, 

 but only a trifling precipitate resulted. The aqueous solution was then boiled, the coagulum fil- 

 tered off, washed with absolute alcohol, and dried over sulphuric acid. This preparation, "1 C," 

 weighed 2.45 grams and contained as follows : 



Coagulated proteid directly extracted from ground oats hy irenlc alcohol (1 C). 



Carbon 52. G6 



Hydrogen 6. 89 



Nitrogen 16. 32 



Sulphur \ 



Oxygen [ 24.13 



Ash ) 



100.00 



Carbon and hydrogen.— 0.3120 gram, dried at 110° C, gave 0.6025 gram C02=52.66 per cent 

 C and 0.1934 gram H20=6.89 per cent H. 



