MH.MOIKS ()!•' TIN': NATI(>NA1- ACADK.MY OF SCIKNi KS. '^5 



Xitroffcn (Kjeltlahl method). — ().rj(i2l jriaiii, diinl ;it lUl' ('., ;,mvo ;iiiiiiioiiiii=10.7r) cc. IK'l 

 (1 cc. lici=0.0()sr).t n;,.;,!,, N) = l(;.;5li per cent. 



Ash could iKit lie (Ictciiuincd for want of material. 



Notliiii^ more could be olitaiticd out of tiic llltiate from 1 C. 



2 and 3 were united autl treated witli MO cc. of l)oiliii;i alcohol of (».!• sp. j,'r. A part dis- 

 solved to a deep-red solution. The residue was extracted afjaiii with hot dilute alcohol and 

 treated in the same manuer as the other preparations. Thus was obtained !t.()5 urams of substaucc 

 '>2 and 3 A," conlaininjj 1.').47 per cent of nitro|:i;en. 



This iHcparation was again treated, first with hot water and then with hot ahohol ofd.O sj). 

 gr., but only a little dissolved and the nitrogen conti'ut was unchauged. 



The alcoliolic liltrate from 2 and 3 A was further examined, but only '2.:i grams of substance 

 with 7.00 per cent of nitrogen were obtained. The long heating in very weak alcohol to which prep- 

 arations 2 and 3 had been subjected had rendered a large share of the proteid insoluble, and it 

 was therefore lost for further piuificatiou. 



4 was treated with oOt) ce. of alcohol of 0.9 sp. gr., in whicli a part was insoluble, " 4 A.'' 

 This was washed witli alcohol and ether, dehydrated with absolute alcoiiol, and, after drying ovcj' 

 suli»huric acid, weighed 8.9 grams. Its analysis follows: 



Coagulated proteid directly extracted from ground oats by weak alcohol (4 A). 



Carbon 53.09 



Hydrogoii fi. % 



Nitrogen 1(>. .^56 



Sulphur) 28.39 



Oxygon S 



ion. 00 

 Ash 3^ 



,.l.,/,._(».5()(i!t giam air-dried (=0.4.555 dried at 110^ €.) gave 0.0010 gram ash =.35 per ceut. 



(Jarhon and hydrogen.— OMMii gram, dried at 110° C, gave 0.8445 gram CO.=52.90per ceut C, 

 and 0.2710 gram ll20=C.91 per cent II. 



Xitrngen {Dumas method).— i).r>i2r,. gram, dried at 110° C, gave 76.12 cc. gas at 9° C. (barometer 

 750.3 mm. at 9° C.)=16.76 per cent, less correction of 0.20=10.50 per ceut N. 



The alcoholic filtrate fiom 4 A was concentrated to small volume, but as a slimy precipitate 

 appeared, which could not be filtered, the solution was mixed with water. This did not cause the 

 substance to separate in a manageable form, so the liquid was boiled, and after cooling the sub- 

 stance deposited as a gelatinous lump. After filtering, the dei)osit, ''4 B," was treated with abso- 

 lute alcohol and with ether and dried over sulphuric acid. It weighed 2.0 grams. Its analysis is 

 subjoined : 



Coagulated proteid directly extracted from ground oats by iccak alcohol (4 B). 



Carlion... 

 Ily<Irogen 

 Nitrogen . 

 Sulphur ) 

 Oxygen J- 

 Ash S 



53.12 

 6.91 

 10. .52 



Mh.—Viivy little. Not determined for want of substance. 



Carbon and hydrogen.— ihSU') gram, dried at 110° C, gave 0.6700 gram CO>=:."..3.]2 i)cr cent 

 C, and 0.2143 gram H2O=0.91 per cent H. 



mtrog^n, I {Dumas method).— () 2r,0:i gram, dried at 110^ C, gave 37.93 cc. gas at 8° C, less 

 correction of 0.7 cc. (barometer 756.3 mm. at y C.) = 10.52 jier cent N. 



