MK.MOIUS «>K TIIK NATIONAI. .\('AI>i;.M Y OT SCIKNCHS. 



of GO per cent alooliol in a rta.sk fitted with a rotnrn condenser. After tlirce lionrs'hoilinfr, aliont 

 one-half had di.ssolvcd. Two liters more of GO per cent aU-ohoI were f.lien added an<l the boil- 

 in<I continned. Oonsiderabh^ proteid .still remained in a fielatinous form on tiie .sides of the 

 tla.sk. The solntion was tiltered olf and 1:!00 cc. of alisolnte alcohol were ponred on the residnal 

 snbstance. This ean.sed the jelly to break up into coarse lumps, whereupon -SOO cc. of water were 

 added, making 2 liters of GO i)er cent alcohol, and tlie boilin;; on the water bath was continued 

 for several hours. Some of the substance dissolved, the liipiid was filtered, and the in.solnble resi- 

 due was treated for many days with absolute alcohol. It was then ground to a fine powder while 

 still moist with alcohol, extracted with ether, and dried over sulphuric acid. This preparation, 

 ''7," weighed 0.1 grara.s. 



The alcoholic .solution tiltered from the insoluble portion was concentrated to one-half and 

 tiltered, while hot, from the largo amount of substance which had separated. The latter resembled 

 raw rubber in a[)|Karance and consistence, but not in elasticity. It was finely minced, and, after 

 having been digested with absolute alcoliol, was pulverized, ti-eated again with absolute alcohol, 

 wa.shed with ether, and dried over sulphuric acid. This, "8," weighed 2(\ grams and was a light 

 powder of yellowish color. Unlike all the other preparations extracted from the oats by weak al- 

 cohol which were analyzed, 8 was still largely " uucoagulated " and soluble in that reagent. 



These two preparations were analyzed with the following results: 



Voagulated proteid directly extracted from (/round oats by weak alcohol (7). 



Ash.— 1.001 grams air-dried substance (=0.9291 gram, dried at 110° C.) gaveO.OOL'3 gram ash 

 =0.25 per cent. 



Carbon and hydrogen.^0.5076 gram, dried at 110° C, gave O.flSGO gram C02= 52.98 per cent 

 C, and 0.3131 gram H.O = 6.S6 per cent H. 



Xitrof/en, I (Dumas method).— O.oOOO gram air-dried (= 0.400G gram, dried at llOo C.) ,aave 

 64.1 cc. gas at S^ C. (barometer 750 mm. at 15° C.) = 16.54 per cent less 0.20 found in blank = 

 16.34 per cent N . 



mtrofien, II (Kjeldakl metliod).-^.5000 gram air-dried (= 0.4G0G gram, dried at 110= C.) gave 

 ammonia z=S.)i5 cc. HCl (1 cc. IICl = .00854 gi-am N) = 16.41 per cent. 



Nitrogen, 111.-0.5000 gram air-dried (=0.4011 gram, dried at 110° C.) gave ammonia = 8.85 

 cc. HOI = 16.39 per cent N. 



Soluble proteid directly extracted from, ground oatu by weak alcohol (8). 



Ash.—l.OOir, grams air-dried (=0.9027 gram, dried at IKP (3.) gave 0.0022 gram asli=0.24 

 per cent. 



Carbon and hydrogen. ^O.iS'2l gram, dried at 11(»= ('., gave 0.9.J.".tJ gram 00^=52.92 jter cent 

 0, and 0.3003 gram Il20=6.93 per cent II. 



