60 



MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



Ash. — 0.6007 gram air-dried left no ash. 



Carbon and hydrogen. — 0.5886 gram, dried at 110° C, gave 1.1.579 grams CO2=r)3.04 per reut 

 C, and 0.3042 gram H20=G.88 per cent U. 



Nitrogen, I {Dumas' mt*7io(7).— 0.5000 gram air-dried (=0.4479 grain dried at llOo C.) gave 60.08 

 cc. gas at 8° C. (barometer 757 mm. at 12° C.) = 15.90 per cent N, less .20 for blank = 15.70 i)er 

 cent. 



Nitrogen, II (Kjeldahl method). — 0.5000 gram air-dried (= 0.4479 gram dried at 110° C.) gave 

 ammonia = 8.05 cc. HCl (1 cc. HCl = 0.00854 gram N) = 15.30 per cent. 



Sulphur.-^.89o8 gram, dried at 110° C, gave 0.1142 gram BaS04= 0.0157 gram = 1.75 per 

 cent S. 



3. EXTRACTION WITH ALCOHOL AFTER TREATMENT WITH SALT SOLUTION. 



(Preparations 10 and 11.) 



Five pounds of oats were repeatedly treated with 10 jier cent solution of sodium chloride until 

 nothing more was dissolved, and after pressing out as thoroughly as possible the residue was 

 twice treated with alcohol of 0.912 sp. gr., being pressed out each time, and the extracts kept 

 separate. The first and second alcohol-extracts were united, evaporated on the water bath 

 until all alcohol had escaped, and the residue then cooled to 10° 0. At this temperature the 

 deposit formed brittle lumps, which, after decanting the solution, were treated with ether and 

 absolute alcohol. The lumps were thus brought into a granular state, in which condition the 

 substance was treated for some time with ether until all fat had been removed. It was then 

 dissolved in hot dilute alcohol, filtered, and evaporated on the water bath to a small volume. On 

 cooling the f)roteid separated in a mass on the bottom of the dish. The aqueous liquid was poured 

 off, and the residue treated with absolute alcohol and ether, whereupon it became brittle and was 

 easily ground to a fine jiowder. This powder was washed with water until it no longer gave with 

 silver nitrate a reaction for clilorine. It was then treated with absolute alcohol and dried over 

 suli^huric acid. In appearance and behavior it resembled in all respects the substance extracted 

 by alcohol after the oats had been treated with water. When analyzed it was found to have the 

 composition stated under 10. 



The third alcoholic extract of the oats was treated in the same way, and the product obtaijied 

 analyzed with the following results (11): 



Sohihle proteid extracted by iceak alcohol from gro^md oats after treating them with niilt solution 



(10 and 11). 



DETAILS OF ANALYSES OF lO. 



Ash. — 1 gram air-dried (=0.9361 gram dried at 110° C.) gave 0.0053 gram ash = 0.56 per cent. 



Carbon and hydrogen. — 0.3443 gram, dried at 110° C, gave 0.6780 gram C02=53. 70 per cent 

 C, and 0.2200 gram H2O = 7.09 per cent II. 



Nitrogen, I (Dumas method). — 0.5000 gram air-dried (=0.4081 gram dried at 110° C.) gave 

 60.47 cc. gas at 8° C. (barometer 772 mm. at 8° C.)=15.80 per cent, less 0.20 for l)lank=15.60 per 

 cent N. 



Nitrogen, II (Kjeldahl method).— 0.5000 gram air-dried (=0.4681 gram dried at llO^ C.) gave 

 ammonia=8.53 cc. HCl (1 cc. HC1=0.00854 gram N)=15.57 per cent N. 



