MKMOlltS OF TIIK NATIONAL ACADKMY OF SCIKNCFS. 



1)1 



Nitriu/cii ///.— 0.5000 },Maiii ii;\\o iiminoiiia=S.5r. <c. I1<"1 = I."..5<t jut ciMit N. 

 Sulphur.— I f,'iam air ilrieil substaiii-e (:^0.'.».!(il },'ram at 110 (".) gave O.lL'.t-' ^'^anl I'.hSO =. 

 O.Ol(il) {,'ram=I.SO \h'v leiit. 



DKTAILS or AX.M.VSKS OF II. 



^Is/i.— 0.8(;80 pram, «lrif(l at 110° C, gavi' O.OOliU pram ash=0.'_'r) jhm- ceiil. 



Carhonand hydroijt'n.—O.MiH) pram, drioil at 110'^ (!., pavo 0.7100 pram C().=r,:i.V2 jur ciiit 

 ('., anil 0.2242 pram 11,()=(!.7S jior ci-iit 11. 



Mtrofien, I (KjehUihl iiirtliod).—0.r>(H)0 pram (=0.4715 pram dried" at I 10 ■ ('.) pavr aiiiiii()iiia = 

 S.m Of. IlCl (1 cc. llCl=0.tK»8r>4 pram N) = 15.r.ll per cent N. 



Xilroilfii //.— 0.5000 pram (=0.4715 pram dried at 110^ C.) pave ammi)nia=::8.55 «i-. HC1= 

 15.41) per eeut N. 



4. EXTRACTION WITH .VI.C'OllOI. AITKK TIJKATMEXT WI'l'lI \VATK1£ ANU SALT-S(JU;T10N. 



(Pr.'liariitioii 12.) 



Five poimds of oats were extracted with water, and tlun with 10 i)er cent saltsolntioii asloiip 

 as anj-thing was removed. Tlie residue was t\yi(e dipested with ak-oliol of 0.9 sp. pr., tlie two 

 alcohol extracts were united and concentrated to a small \ olume on the water hath. xVfter cool 

 inp, the substance which deposited was filtered out and dissolved in dilute alcohol. The filtered 

 solution was evaporated until nearly all the alcohol was exjielled, and finally poured into cold 

 water. The abundant precipitate thus produced settled rapidly, forming a pasty mass on the 

 bottom of the dish, from which the water could be completely decanted. By treatment with 

 ether and absolute alcohol the substance was rendered dry and brittle, and was easily pround to 

 a yeUowish powder. This was transftared to a llask and dipested for twenty-four hours with a 

 mixture of absolute alcohol and ether, then washed with ether to remove the alcohol, and dried in 

 the air. The product weighed 12 prams. After dissolving this preparation in dilute alcolnd and 

 evaporating to a small volume on the water-bath, the concentrated solution was poured into a liter 

 of cold distilled water. Tiie substance thus precipitated was washed by decantation with distilled 

 water nntil all chlorides had been removed, then treated with absolute alcohol and ether and dried 

 over sulphiu-ic acid. This preparation was found to have the following composition : 



Soluble jjroteid extracted by ircak alvohol from ground oats after treating them with water and with 

 .salt.sidiition (12). 



^4,s/,._o.7593 gram, dried at 110° C, gave 0.0008 gram ash=0.11 per cent. 



Carbon and hydrogen.— ^.2S50 gram, dried at 110° ()., gave 0.5595 pram COi=.-)3.58 per cent 

 C, and 0.1830 pram H,0=7.15 per <ent 11. 



Xitrogen, I {Dumas method).— OAWS pram, dried at UOOC, gave 56.95 cc. N at 18o O. (barom- 

 eter 764.5 mm. at 22^ C.) =15.81 per cent. 



Mtrogen, If {Kjrhlahl method).—l.Ols:i prams, dried at IK)^ C, pave ainmonia = 19.(i.{ cc. IICI 

 (1 cc. HC1=0.0(»S22 pram N) = 15.84 per cent. 



,Sm//>/i«»-.— 0.9G82 gram, dried at IKP C, pave 0.1218 pram I{aSO4=«.01<i"-'5 pram S = 1.74 per 

 cent. 



