MKMOIUS OF TllH NATIONAL ACADKMV OF SCIIiNCKS. (,;) 



To sum up, Wf liavii in tlu> aqufous i^xtiiU't dfllie iml kernel tlie following,' Ixiilies: 



1. An arid aibnniiii, preeipitut^'d by exaet lUMitralizatioii of the, extract. 



2, Olio or iiioro globulins, prtM-ipitatcd froiii tlie iieittrali/.ed extiaet by Kodiiiiii elibiride and 

 acetic aeid; also precipitated by saturation witli auiinoniuni Hulpiiat^t; reniainin{^ insuluble in 

 water on dialysis, beinji^, as it would appear, by the a<'tion of the water cr)iivertetl into an "albu 

 uiinate." 



ii. A prottom; reinainin'r in solution atler dialysis of the ainnioninni-sulphate ]irc(ipiiate. 

 This l)ody exists also in the dialy/.ed solution of the aninioniuni suljjhate precipitate frurn the 

 sodiuni-ehloride extract. 



V. — Pkoteius Extracted by Gold Sodhim Chloimdk Solution. 



1. DIRIX'T KXTRACTION WITH SALT .SOLUTION. 

 (I'ropanitioiiH 13 and 14.) 



Freslily-','ronnd oats, extracted with 10 per cent brine of sodium chloride* at 15'^ to 20'=, gave a 

 brown solution which, when tittered clear, coagulated as follows: Heated to 42^ C, a very slight 

 turbidity formed, whicb increased very slowly up to 57°, at which temperature it was still sliglit. 

 Above this point it iii(rea.scd more rapidly, at (Jl° tlie .solution being opaque; at 72"^ tlocks 

 formed. Ileated at 7.5^ some minutes atul then tiltered, the tiltrate became turbid again at 

 70^, the turbidity increasing somewhat up to 87°; from 87° to '.»0° the increase was more rapid, but 

 the amount separated at 90° was small. The solution heateil to 91)° and filtered, became turbid 

 again at 8."»o, with little change up to 97°. Ileated to boiling for a short time and filtered, the 

 filtrate gave abundant precipitate with acetic acid. 



Dilute tujetic or hydrochloric acid yields a large precipitate in the briue extract, which is insolu- 

 ble in a slight excess of the acid. Either saturation with sodium chloride or dilution with water 

 throws down a copious precipitate. The precipitate produced by water, on standing in the dilute 

 salt scdutiou two days, becomes insoluble in 10 per cent .sodium chloride brine and in one-half per 

 cent .sodium carbonate solution. 



Saturation witli ammonium sulphate completely precipitates the proteids trom this solution in 

 10 per cent sodium chloride brine. 



Fivepimnds of freshly ground oats were treated twice with 10 i)er cent sodium chloride solu- 

 tion, and after filtering, the extract was s:iturated with conunercial — but perfectly ueutral — am- 

 monium sulphate, which gave a dark greenish color to theextracteilsubstames without otherwise 

 atfccting their properties. The bulky precipitate was filtered oflF, susi)eudcd in water and dialyzed 

 fourteen days, uutil nearly free from sulphates. 



A heiivy i)rcciiiitate remained undissolved. This was filtered out, and the filtrate and precip- 

 itate were .scpanitely examined. 



Filtrate. — When heated very slowly in a test-tube, in the nmnner previou.sly described, the 

 solutiou was found to become turbid at 58° (;.; at 70° C. flocks were formed. Ileated to boiling 

 and filtered, the filtrate gave a strong rea((ti(m with Milieu's reagent. The entire .solution was 

 therefore concentrated to a small volume at 40° C, and dialyzetl until all salts were removed. 

 The coagulation point of the solution was again taken and found to be the same as before; tur- 

 bidity at 58° C; flocks at 73° C. The solution was strongly colmed, appearing almost black by 

 reflected light, and by transmitted light a greenish brown. With sulphate of copper and caustic 

 potash and with Millou's reagent it gave good reactions; was not attected by very dilute hydro- 

 chloric acid, but was precipitated by stronger acid in the cold. Fehling's .solution sutiered no 

 reduction, either before or after the action of a<-id. This .solution was then evai)orated on a plate 

 below 50° C, and yielded about (J grams of a very brittle, greenish-black, shining substance, very 

 soluble in water, from which alcohol of 0.9 sj). gr. dissolved some i)roteid. 



It is .seen that when oat~s art; extracteil with 10 per cent sodium chloride .solution, a substan(!e 

 soluble in water andcoagulating at .'•8° to 7.3° C. is formed, which does not exist in the oats origi- 

 nally, for the aqueous extract, when treated in the same way, yields no substance coagulable even 

 ni)on boiling. 



"The soiliiiui eliloriili; iMuployeil Wiw thu " Dijiiiioiiil Crystal Tablo Salt," ICiireka Salt t'liiiipany. Xew York, 

 whirli i.s 'Tomarkalily fri'O from iniiiiiritu-s." 

 S. Mis. 169 5 



