MKMOIKS OF Tin: NATIONAL ACADKMY OK SCIKNCKS. 

 ()<il (ilohuUn.— Firxt direct >jtni<tii,ii with in p, r mit suit s,iliiti«n (13). 



(57 



^.s/i.— (i.7!t;57 fjiam gave 0.0015 {jrain a8li=0.20 per cent. 



Carbon and hydrogen, /.— 0.35G1 grain gave 0.<i822 grain C02=52.23 jicr (ciit C. ami (».2;5(M» 

 gram HjO^T.lo \wv cent H. 



Carbon and hi/droaen, //.— 0.4584 gram gave 0.8778 gram <'()j=52.22 per cent (', and 0.2883 

 gram H.O=().09 per cent II. 



Xitrogen, I {Dumax mi-thod). — 0.0225 gram gave 00.85 cc. nitrogen at 18"^ ('. (barometer 7fi.').5 

 mm. at 22° ('.) = 1(!.92 per cent N. 



Xitroffen, II(Kjeldnhl method).— OftlCO gram gave ammonia= 1 1.05 cc. IICl ( 1 cc =0.00822 gram 

 X)=17.07 per cent N. 



Xitro(ien, /JJ.— 1.0476 gram gave ammonia=21.54 cc. HC1=1C.1»0 per cent X. 



iS«/iJ/(«/-.— 0.8022 gram gave 0.0513 gram BaSO4=0.00705 gram S=0.88 per cent S. 



A second preparation of globulin was as follows: Five pounds of ground oats were digested 

 with 8 liters of 10 per cent sodium chloride solution for forty-eight hours, and pressed out. The 

 residue was again subjected to the same treatment. The two extracts were uniterl, filtered, the 

 clear filtrate saturated with pur^ ammonium sulphate, and the jnecipitate thus produceil was 

 filtered off. This i)recipitate was found to be completely soluble in 10 per cent salt solution, save 

 a .slight turbidity, which was readily cleared up by very dilute sodium carbonate solution. It was 

 therefore removed from tiie filter, suspended in water, ami dialyzcd for eleven days, whereby the 

 salts were nearly all removed. The precipitate was filtered ofl', and the filtrate and precipitate 

 were separately examined. 



Filtrate. — The clear brown filtrate was evaporated to dryness at 50^, leaving an amber-brown 

 instead of a greenish-black residue, as was obtained from the first salt extract. 



The properties of this residue agreed exactly with those from the first salt extract, the 

 difference in color being due to impurities in the ammonium suli)liate used in saturating the first 

 extract. 



rrecipitate. — The precipitate in the dialyzer was found to remain almost entirely soluble in 

 salt solution, the trifling residue not taken up by that solvent yielded to dilute sodium carbonate, 

 and when this solution was neutralized with a slight excess of acetic acid the separated substance 

 dissolved in solution of common salt. 



The dialyzed precipitate was therefore filtered, washed by decantation with water, alcohoi, 

 ether, and absolute alcoliol, and dried over sulpliuric acid. This body had ail the itrojuMties of 

 the one similarly prepared and already described (13). Twenty grams of substance were thus 

 obtained, and by extracting the oat residue with 10 per cent .sodium chloride solution, until no 

 more proteid was taken up, and i)roceeding as Just detaileil, ten grams more of globulin were 

 secured. Tlie yield was accordingly 1.3 per cent of the air dried oats. Tlie analyses of this 

 preparation here follow : 



