(5y MEMOIES OF THE NATIONAL ACADEMY OF SCIENCES. 



Oat globulin. — Second direct extraction with 10 per cent suit nnlutiiiii (14). 



^,5^. — 0.8060 gram gave 0.0017 gram ash=0.21 per cent. 



Carbon and hydrogen, I. — 0.2894 gram gave 0.554.") gram 002=52.28 per cent 0, aurt 0.1883 

 gram H20=7.23 per cent H. 



Carbon and hydrogen, 77.— 0.2190 gram gave 0.4200 gram 002=52.33 per cent C, and 0.1412 

 gram H.O^T.l? per cent H. 



Nitrogen I (Dumas method). — 0.5314 gram gave 77.1 cc. nitrogen at 18° C. (barometer 703.5 

 mm. at 22° C.)=16.78 per cent N. 



Nitrogen II {Ejeldahl method). — 0.4732 gram gave ammonia=9.73 cc. HCl (1 cc.=0.00822 gram 

 nitrogen)=16.90 per cent N. 



Nitrogen III. — 0.9973 gram gave ammonia=20.64 cc. HC1=17.01 per cent N. 



*(ip/mr.— 0.8400 gram gave 0.0547 gram BaSO4=0.007513 gram S=0.89 per cent S. 



2. EXTRACTION WITH SALT SOLUTION AFTER TREATMENT WITH ALCOHOL. 

 (Preparatiou 15.) 



Since digestion of oats first with water or with salt sohitiou altered the proteid solnble in 

 alcohol, it was thought possible that by preliminary treatment with alcohol a body might be 

 obtained soluble in salt solution and yet ditterent from the one extracted directly by thart; solvent. 



To test this hypothesis, 3 pounds of fine ground oats were three times extracted, in the cold, 

 with 6 liters of alcohol of 0.9 sp. gr. After pressing out as thoroughly as possible, the residue was 

 treated three times with 10 per cent sodium-chloride solution, the solvent each time being allowed 

 to stand in contact with the oats for twenty-four hours. The first and second extracts were united, 

 filtered, aud saturated with sodium chloride. The precipitate was filtered out and found to be 

 but partly soluble in 10 per cent brine. It was therefore dissolved in solution of sodium carbonate 

 of one-fourth per cent, and the filtered liquor was exactly neutralized with acetic acid, which 

 separated the proteid as a white precipitate. After washing thoroughly, by decantation with 

 distilled water, with dilute alcohol, with ether, and finally with absolute alcohol, the sub.stauce 

 was dried over sulphuric acid. As it weighed only 2.1 grams, the greater part of the proteid in 

 the oats, soluble in salt solution, had evidently been converted into an "albuminate." 



Oat globulin extracted by 10 per cent salt .solution after treatment with alcohol, dissolved in one-fourth 

 per cent sodium-carbonate solution and precipitated hy acetic add (15). 



Carbon 



Hydrogen . 

 Nitrogen .. 

 Sulphur .. 

 Oxygen . . . 

 Ash 



16.82 

 0.57 



6.94 



16.85 

 0.57 

 23.16 



