.mi;m()II;s oi-- iiii: n.\ tional .\<'.vi)i;.my ni- s("ii;.n('Ks. 



69 



.•.;t!l),«T<fllt «', MIkI (l.l'.l'.t.t ;r,jim 



Anh. — ()..?77.{ Kiaiii gavi" 0.(M>0(i ■jiam a.sh — 0.17 i»cr rent. 



Carbon and hydnxjeti. — 0..US7 }i>a"> fli'vi' «M>I21.' f;iiiin CO, 

 H.O = G.!)3 per cent 11. 



S'itroijen [DnmaH »/*<7/i«(/).— O.-WJli hy-.ww •favc 17.17 (•<■. nitniKfii at IH ('. (l.aioiiictcr 

 mm. at 28'= C.) = 1(J.82 j,or ci'iil N. 



Sulphur. — ().74.{."') {jram {Ci»vi> (Miidl liaSO, = (i.ddlL'KI ;;iaiii S = 0.."i7 ptr tent S. 



VI. — Pkoteids Extractki) hv Wkak Alkali Solutions. 



1. EXTRACTION liV TWO-TENTIl.S I'EK CENT POTASH SOLUTION AFTER TREATMENT oK THE (iUOINI) 

 (UTS WITH ALCOHOL. 



(Preparation 16). 



Another extraetion was made in nearly the same inaniu'r as that em]»loye.d by Kreusler in 

 preparing his "oat legumin:" Five pounds of freshly tjround oats were treated in the cold with 

 alcohol of ().!> sp. gr. a.s long as any proteid was removed. The residue was digested for some 

 time with 7 liters of two tenths per cent i)otash solution. The whole wits then thrown on a sieve 

 and the turbid percolate allowed to stand for twenty four hours. 



The dark-colored solution was siphoned olf, let stand aiiotlier day, decanted from the .slight 

 sediment, and then luecipitated with very dilute acetic a<id. After a day the substance had so 

 far settled that about two-thirds of the liquid could be decanted. To the remainder alcohol was 

 added until the solution had a sp. gr. of 0.9.}. On further standing the i)rci*ipitatt! depo.sitcd and 

 wa.s transferred to a filter. It was washed with stronger alcohol, removed from the paper, and 

 dissolved again in two-tenths per ceut potash solution. A turbid liquid resulted which could uot 

 be tiltered until a little sodium chloride had been added, which precipitated some of tlie dissolved 

 proteid. The precipitate was .soluble botli in addition-.il potash solution and in brine. More salt 

 was therefore added; the uearly clear solution was then filtered, which process lasted for about a 

 week. Decomposition was entirely prevented by the use of tiiyuu)l. The filtrate obtainetl each 

 day was precipitated with dilute acetic acid; the precii)itate was washed on a filter with water, 

 then with dilute aiul afterwards with strong alcohol, and was finally trau.sferrcd to a flask filled 

 with absolute alcohol. When the filtration was completed and the united preci]»itates had been 

 digested with absolute alcohol, they were treated with ether and again ^vith ab.solute alcohol, and 

 lastly dried over sulphuric acid, yielding preparation " 16." 



Oat proteid extracted liy tiro tntlhn per 



"lit potash xolutio 

 alcohol (16). 



at'ter trrutmeiit of the (/round ontn with 



Agh. — 0.7.J73 gram gave 0.0040 gram ash = O.OIi per cent. 



Carbon and hydrogen, I. — 0.3006 gram gave 0.7005 gram 

 giam Il.O = f...s7 per cent II. 



Carbon and hydrogen, II. — 0.-ir)23 gram gave 0.86.")1 gram <] 

 gram II2O — 0.74 per cent II. 



Nitrogen, I (Dnman method). — 0..!.'>77 gram gave .''>1.41 <-c, 

 mm. at 22'= C.) = 10.52 per ceut N. 



Nitrogen, II (Kjeldahl method). — O.'AYM gram gave aniii 

 0.00822 gram N) = 10.48 per cent N. 



Nitrogen, TIL — 1.0412 gram gave ammonia = 20.77 cc. HCl = 16.40 jior cent N. 



6'«//>/i«r.— 0.8670 gram gave 0.0510 gram I'.aSO, = 0.0070 gram S = O.Hl \m- cent S, 



'O, = .-.2.11 per cent C, and 0.2265 

 ("O. = .-CMS per cent V, and 0.2740 

 nitrogen at 1S= ('.(barometer 7.58.7 

 inia = 10.1 cc. Iin (1 cc. 1IC1 = 



