MEMOIKS OK TlIK NATIONAL A( AKK.MV OK SClKNCliS. 



71 



not result in the extraction (ilnmcli if any more ;;li>l)niin tlian was ol.laimil when a cold salt soln 

 tion was oiui)loyc(l. 



The trials next to lie described have an ini]iortant hcarintion the alterations ol' protcids which 

 take place in the ]»resencc of water. 



2. UlUKCT KXTKACTION 



nil; (!1{(»1ND OATS Willi T\V( 



(rroiiaratioii 17) 



I'DTASM SOM ri«»N. 



One hundred grains of freshly-ground oats were tieatcd with 5(»0 cc. of two-tenths per cent 

 l>otash solution. The niixture, after standing some time, was found to be neutral to litmus-paper. 

 It was then strained through a coarse cloth to remove the husks, and the residue was treated with 

 100 ce. of two-tenths per cent jiotash solution and squeezed out nearly dry. The solutions and 

 washings were united and 100 ce. two-tenths jier cent potash solution added thereto, making 700 

 cc. in all. The liquid was then faintly alkaline fo litmus. On standing, the insoluble matter set 

 tied out; the solution was decanted, filtered, and the residue treated again with two-tenths per 

 cent potash solution. The lirst potash extract was very dark brown in color, the second much 

 lighter. A third extract contained very little proteid. The first and second extracts were united, 

 filtered, and precipitated with acetic acid added to acid reaction, washed thoroughly with water, 

 alcohol, and ether, and dried over sulphuric acid. Th.e preparation weighed 7.8 grams. A por- 

 tion treated with two tenths per cent potash lye was completely soluble, giving a perfectly clear 

 solution. The rest of the pi ei)aration was treated with hot alcohol of 0.9 sp. gr. which took up a 

 little jiroteid. It was washed with hot alcohol of 0.0 sp. gr. until nothing more was removed, then 

 with absolute alcohol and finally with ether, and was dried over sulphuric acid. After drying 

 at 110° C. the substance was anaJysed with the following results : 



Oat proteid directly extracted with two-tenths per cent potash solution (17). 



Ash.— (L'OGS gram, dried at UO^ C. gave 0.0071 gram of ash=1.00 per cent. 



Carbon and hydrogen. — 0.2G51 gram gave 0..')148 gram C02=52.!I6 jier cent <', and 0.16.'i4 gram 

 n.,0=6.93 per cent H. 



Xitrofien I {Kjeldahl method).— 0.13i):> gram gave ammonia=l-1.6 cc. Uf'l (1 cc.=(l.00822 gram 

 N) = 10.2;} per cent N, 



Nitrof/cn II (Pumas method). — 0.4285 gram gave 50.82 cc. nitrogen at 15o C (barometer ~'>l.r> 

 mm. at 21° C".) = l(!.10 per cent N. 



Snlphur.-^.rtlQG gram gave 0.037;i gram Ba 80^=0.00512 gram 8=0.08 per cent S. 



3. IJIRECT K.\1'KA( TlON OF PHH (iKOUNI) O.VTS WITH .SODIir.M CARBONATK SOLUTION, 

 (rrcparatioii 17 A. ) 



One hundred grams of ground oats were digested with a 1 per cent sodium carbonate solu- 

 tion, the extract was hltered and the .sodium caibonate converted into bicarbonate by trea'tmeut 

 with carbon dioxide. The solution was then saturated with ammonium suli)hate and the heavy 

 precipitate filtered off. This jjiecipitate was washed with a saturated solution of ainmoiiiuin sul- 

 phate until the washings no longer reactted alkaline. The precipitate was then thoroughly ex- 

 tracted with Itt per cent .salt .solution until all globulin was separated (see .\ p 551 and after 



