72 



MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



■washing out the sodium chloride the residue was extracted with warm 80 per cent alcohol until 

 all the proteid soluble in that liquid was removed. 



The residue was then found to be quite insoluble in one per cent sodium carbonate solution 

 and was therefore treated with two-tenths per cent caustic potash solution, filtered, and the dis- 

 solved proteid completely precipitated by neutralizing with dilute acetic acid. After washing this 

 substance with water, alcohol, and ether, and drying at 110° C. its composition was as follows: 



Oat proteid extracted from grotmd oats by sodium carbonate solution (17 A.) 



Ash. — 0..5661 gram substance dried at 110° C. gave 0.0041 gram ash=0.72 per cent. 



Carbon and hydrogen. — 0.32G9 gram dried substance gave 0.G383 gram C02=53.25 per cent C. 

 and 0.2090 gram Ha = 7.13 per cent H. 



Nitrogen, I. — 0.3637 gram dried substance gave 49.33 cc. N. at 13° C. (barometer 7G1.5 mm. at 

 250 C.)= 15.99 per cent N. 



Nitrogen, II. — 0.3916 gram dried substance gave 52.95 cc. N. at 13° C. (barometer 759.2 mm. at 

 23° C.)=15.89 per cent N. 



Sulphur. — 0.5000 gram dried substance gave 0.0295 gram BaSOj =0.0041 gram S=0.81 per 

 cent S. 



If this analysis is compared with that of preparation 17, which was obtained from ground oats 

 by direct extraction with two tenths per cent potash solution, it will be seen that the two agree 

 closely. 



Carbon 



Hydrogen . 

 Nitrogeu . . 

 Sulphur .- 

 Oxygeu . . . 



4. EXTRACTION OF THE GROUND OATS WITH WEAK POTASH SOLUTION AFTER ONE HOUR'S TREAT- 

 MENT WITH WATER. 



(Preparation 18.) 



One hundred grams of ground oats were next treated with 800 cc. of water, the mixture was 

 passed through a cloth to remove husks, and the residue washed with 200 cc. of water. The liquid, 

 with suspended starch, etc., was made up to the volume of a liter, and, after the oats had been 

 for an hour in contact with the water, an equal volume of two-tenths per cent potash solution was 

 added and intermixed. 



After standing over night the strongly-colored solution was decanted fi-om the residue, filtered, 

 precipitated with acetic acid, the precipitate washed with hot alcohol until nothing more could 

 be removed, and then with absolute ah-ohol and ether, and dried over sulphuric acid. This 

 preparation weighed 4.25 grains. The residue was treated a second time with two-tenths per cent 

 solution of potash and very little more proteid was extracted. After drying at 110° C. this 

 preparation was analysed with the following results: 



