Mi;.M()ll;s (IK TIIK NATIONAL ACADEMY OK SCIENC'KS. 



Tlio resuhii" of tlw oats was t<xtratte(l a scrond time in tlu^ samo iiiaiiiitT and the sulntion 

 was at onco saturated witli aninioniiini sulphate. Tlie iirecipitato thus jiiodiKi'd was washed 

 directly with 5()per<ent aieohol; Init, since the substanee «lissrtlved on washing willidilMlr aictdiol 

 as lonj; as niueh annnoniuni sulphate remained, the final yield was oidy 2.0 {jiams. 



The iiioiliut obtained tVoin the solntion of the first ammonium sulphate i)reeii)itate in hot 

 brine has the followiiit,' ju-operties: Under the microscope before wasliinp, it appears to be per- 

 fectly homogeneous and toeonsist entirely of spheroids about (t.ttl nun. in diameter. After washing 

 and ilrying it forms a dense, suowwhite powder. In cold distilled water it diswdves to an oi)ales- 

 ceut solution which gives a heavy precipitate on the addition of a liitle sodium chloride; a little 

 more sodium chloride precipitates the a(pu'(ms solution almost eomi)let^^ly, and on iuldition of still 

 more salt this precipitate is again dissolved. 



Hot distilled water dissolves this proteid completely to a perfectly clear solution from which 

 a considerable part of the substance deposits on cooling. 



Dilute acetic acid ahuie gives no precijiitate in the aqueous solution; additionof a little salt 

 together with the acid precipitates it. The precipitate thus produced is soluble in alcohol of ().!» 

 sp. gr. 



When treated with hot dilute alcohol the substance melts and remains suspended in tlic solu 

 tion in transparent droplets; adiiition of a little salt produces no change, but addition of a little, 

 acetic acid gives a clear solution. This solution in alcohol, on cooling, forms a very bulk.\ , trans- 

 parent jelly. On evaporation, the substance separates as a skiti on the surface, which is readily 

 soluble again in dilute alcohol of 0.0 sp. gr. 



On analysis the composition of the dried spheroids was found to be as follows: 



Oat proteid extracted by sodium-chloride solution at 63° C. (20). 



.l.s//. —0.4004 gram substance, dried at lioo C, gave 0.0009 gram of ash=0.10 per cent. 



Carbon and liydrof/en, I. — 0.3639 gram dried substance gave 0.6955 gram C02 = 5li.i;5 per cent 

 C". and 0.2270 gram 11,0 = 6.03 i)er cent II. 



Carbon and hydrogen, II. — 0.4728 gram dried substance gave 0.9036 gram C02 = 52.1(J percent 

 ('. and 0.298.1 gram II,0 =7.02 per cent H. 



Xitrogen, I {Kjcldahl method). — 0.7768 gram substance gave ammonia = 16.75 cc. IICI Sol. 

 (1 cc. = 0.0OS22 gram N) = 17.72 ])er cent N. 



Xiirotjcn, II {Dumas method). — 0.40.S() gram dried substance gave 62.36 cc. N at 15° C. (barom- 

 eter 762 mm. at 21° ('.) = 17.87 per cent N. 



Sul2)hur, I. — 0.7841 gram dried substance gave 0.0403 gram r.aS04 = 0.0004 gram S = 0.S1 per 

 cent S. 



Sulphur. //.— 0.,S67O gram dried substance gaveO.0460 gram lJaS(), = 0.((003 gram S = 0.7.{ j.er 

 cent S. 



The fact that this substaiuje sei)arated from warm sodium chloride solutions so readily in tlu? 

 form of spheroirls indicated that, under projier condition.s. it might be obtained in recogni7,al)le 

 crystals. After a very large number of attempts, which resulted only in the production of sphe- 

 roids, a jiortion of that substance, which had deposited on dialyzing the mother liiiuor from 20, 

 was conrerted into a mi.xture of splieroids and ciystals. The latter were about 0.1 mm. in their 

 greatest diameters and to all appearance had distinct rhombic faces, but they were not perfectly- 

 developeil. 



