MKMOIK8 OF TUH NATIONAL ACADKMY OF SCIKNlKS. 



7t) 



.Uh. — 0.(>7.")!» fjram .substance dried at 110' C. gav*^ O.OOO'J ciiiiii of ash = 0.03 per cent. 



Ctirhon nmlhijihiujin. — (».4(!10 gram dried substance gave O.SS.i? {,'ram OO.. = 513.2H p<'r (ciit ('., 

 an<l 0..$2J0 {jiain 1I..O = «.!>« per cent II. 



Xitronni, I (Pumas mt'thod).—0:Mli nviun dried substance gave 52.1.") rr. N. at l.P<!. (baroni 

 eter 7(;s.2 mm at '2:r i\) = 17.S(i per cent N. 



yitrtujcn, II {Kjriiliilil inrlhoii). — I.OIOS fjram dried suhstaiice ^ave amiiiniiin = L'l.S cc. IICI 

 Sol. (Tec. = (l.OOSL'J gram N.) = 17.7.t i>er cent N. 



Sulphur.— OJti'ja gram dried sub.stance gave 0.0380 gram IJaSO, = 0.0051.' gram S = 0.i!!t jtcr 

 cent S. 



In all respects .save one this .substance behaved exactly like the globulin obtained l).v extract 

 ing ground oats with hot sodiumclihuide solution. The latter proteid dis.solved to a turbid solu 

 tion in cohl. anil to a perfectly clear solution in hot, distilled water. So far as the usual methods, 

 of analysis show, the two bodies are identical in composition, as is seen from the following ligures: 



Another preparation of this substance was made by dissol^ng a second portion of the '' ;dbn- 

 minate" in 2 per cent sodium-carbonate solution, precipitating with carbon dioxide, washing 

 thoroughly with water, dissolving in brine, and diluting the .solution with a large amount of water. 

 The fiual snow-white precipitate was (iltered off, and after washing with alcohol and ether weighed 

 11 grams. Its properties are the same as those of 21, except that it is slightly soluble in hot dis- 

 tilled water. Its composition as determined by analysis here follows : 



Oat proteid derived from sodium carbonate — Solution of the " albuminate^' (22). 



yl.s/,._()..-,437 gram substance, drie<l at 110° C, gave 0.0007 gram ash =0.13 per cent. 



f'arhoii and hi/dt-oi/cn. — 0.57S9 gram dried substance gave 1.1030 gram C02=51.98 per cent C, 

 and (t.3(J()'.» gram II.O ^(J.!)2 per cent II. 



Nitrogen. — 0.4230 gram dried substance gave 03 cc. N at 13° C. (barometer 773 mm. at23=' 

 C.)=17.83 per cent N. 



The filtrate from the precipitate produced by carlion dioxide in the preparation of the pre 

 ceding sub.stance (22) was diluted with an equal volume of watei, further- treated with carbon 

 dioxide for about three hours, and allowed to .stand over night. The resulting jjrecipitate was 

 Qltered off and washed with water, alcohol, and ether. Five grams of substance having the same 



