82 



MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



Crystallized 0(tt-Proteid, derived from 21. 

 (Preparation 26.) 



Carbon .. 

 Hydrogen 

 Nitrogt'U . 

 Sulphur - . 

 Oxygen - - 

 Ash 



52.23 

 7.0b 



17. Wl 

 0..52 



17.82 

 0.52 

 22.33 



Ash.— 0.3974: gram substance dried at 110° C. gave 0.0002 gram ash =0.05 per cent. 



Carbon and hydrogen. — 0.2866 gram dried substance gave 0.5489 gram C03=52.23 per cent C. 

 and 0.1828 gram H2O=:7.08 per cent H. 



Mtrogen.— 0.3123 gram dried substance gave 48,31cc. N at 17° C. (barometer 757°"". at 21° C)= 

 17.82 per cent N. 



Sulphur. — 0.3598 gram dried substance gave 0.0135 gram BaSO4=0.0018 gram S=0.52 per 

 cent. 



CrystaUzed oat globulin, derived from 21. 



Carbon.. 

 Hyflropei 

 Nitrogen 

 Suljihur 

 Oxygen . 



Ash 



0. 53 : ( 



7.08 

 17.82 



0..52 

 22.33 



No chlorine could be detected in these crystals. It is thus shown that the crystallized globu- 

 lin, except for its .slightly lower content of sulphur, has the same composition as the mother sub- 

 stance, and agrees in all respects with the globulin obtained directly from ground oats by extrac- 

 tion with hot salt solution, except that it is not soluble in water and contains a little less .sulphur. 



It is further to be noted that the methods of preparation were suited to fractionally separate 

 the substance into its component parts, had it been a mixture of two or more globulins. It seems 

 improbable that the sodium chloride, which Griibler found in his crystallized globulin from squash 

 seed, was anything more than an accidental impurity. The ash of Griibler's preparations con- 

 tained traces of the various salts present in the solutions from which they separated, and there 

 appears to be no good ground for Griibler's assumption that the crystallized proteids are definite 

 compounds of globulin with sodium chloride or with other inorganic bodies, since the content of 

 total mineral matters is variable, and, as in my preparations 24 and 26, sometimes amounts to 

 but .05 per cent of the proteid. 



X.— Globulin Obtained by Direct Extraction of Ground Oats with Sodium Car- 

 bonate Solution.* 



One hundred grams of ground oats were digested mbh a 1 per cent sodium carbonate solu- 

 tion, the extract was filtered clear, and the sodium carbonate converted into bicarbonate by treat- 

 ment with carbon dioxide. The solution was then saturated with ammonium sulphate and the 



* This extraction is the same as tliat described ou page 71, 

 I specially considered. 



liiere the iiroteid iusoluble in sodium chloride sohition 



