mi-:m()ii;s of tiik national acadk.my of sciencks. 



83 



lioavy precipitate tiltercil otV. Tliis ]irocipitate wa.s WMslnd willi a satiiratcd solution of amnio 

 niiun sulphate until tlie wasliiug.s no loufjer reacted alkaline Tlie precipitate was tiien extracted 

 thoroujilily with .salt .solution and this extract dialy/.cd. After removal of the salts by dialy.sis, 

 the protoid, which had sei>arated, was tillered olV and washed with alcohol and ether. It weijched. 

 air-dry, Ih grani.s, bciniLr, therefore, about I A iter cent of the oat kernel. Dried at 1 H)^ C, for analysis, 

 it was fouud to have the following? coruitosition: 



Oat proteid cvtrdctnl from >ir<iiinil ottts hi/ kikUi 



III rarhoiKitc 

 'liitiiin. 



solution mill siiluhle in hoiHuiu chloride 



(Preparation 27.) 



.l.s7(.— 0.2652 gram substsince, dried at 110° O., gave 0.0007 grain ash=0.26 per cent. 



Ctn-bon and /(y«?/-0(/eH.— 0.2296 gram dried substance gave 0.4350 gram 002=51.68 per cent C 

 and 0.142!) gram 11,0=6.01 per cent II. 



Nitioficn, /.— 0..'3441 gram dried substance gave 52.15 cc. N at 13° C. (barometer 764.0 mm. at 

 2.>o C. = 17.92 per cent N. 



mtroffen, //.— 0.3294 gram dried substance gave 50.14 cc. N at 14^ C. (barometer 762.0 mm. 

 at 23° C.)=17.91 per cent N. 



XI.— SuMMAUY OF Analyses of Oat Globulin. 



The agreement in the analyses of tlie c^ight different preparations of oat globulin extracted by 

 hot salt solution (20) or obtained from '> albuminates" (21-27) is seen from the following table: 



The writer has nowhere found any de.seripticm of a vegetable proteid like this. Although its 

 proportion of carbon is the same as that of the globulin extracted from oats by cold-salt solution, 

 it nitrogen-content is 1 per cent greater. It contains nearly 1 per cent less of nitrogen, about 

 .1 per cent more of carbon, and but i as much sulphur as Chittenden and Hartwell* fouud in the 

 glubiilin or "vegetable vitellin" of S(|ua8h seeds. 



JJarbieri's analy.sis of squash-seed globulin agrees fairly well with these analyses of the crys- 

 tallized oat globulin, but carbon ami sulphur are somewhat lower, nitrogen higher, and as regards 

 .sulphur and nitrogen his analysis dilfers materially from the later ones of Chittenden and Hartwell. 



Griibler's earlier analy.scs of sriuash seed globulin, which gave 53.21 per cent of carbon and 

 19.22 iier cent of nitrogen, were evidently incorrect, and in a recent publication t this cliemi.st 



•Journal of Pbysiolojiy, xi, 

 t Journal fiir I'raktisiho Ch( 



|.p. .137-tlO. 

 uiiu XLiv, p. 369. 



