434 MEMOIES OF THE NATIONAL ACADEMY OF SCIENCES. 



Colonies hi gelatin. — About the lourtli day the surface colonies are rounded, pinkish expansions,, 

 paler at their margins and usually ill defined in outlines. They may be slightly sunken in the 

 gelatin and may be li mm., more or less, in diameter. Under a low power the surface colonies are 

 seen to be matted masses of thin, irregular, and wavy filaments, rather dense, and of a reddish 

 tint toward their centers, but growing less thick and losing the red color toward their margins, 

 which are ill defined (see PI. I, fig. 1). Probably in younger colonies their margins are sharply 

 defined and wavy in outline; but this condition changes when the slow liquefaction has begun and 

 the colony sinks somewhat in the gelatin. 



The deep colonies are rounded, reddish, granular, and have a slightly uneven margin. Fuder 

 some circumstances they may appear to be made up of closely packed filaments, and may be seen 

 to have finny outlines. 



Gelatin .slant. — A narrow reddish stripe, rather dense. The gelatin is slowly liquefied beneath 

 this and to some extent on each side, and more or less of the growth slips downward. 



Gelatin stab. — Thin, flat, circular pinkish expansions, several millimeters in diameter. Faint 

 growth along the line of inoculation. Slow liquefaction beneath the surface growth in funnel 

 form. 



Acid gelatin. — Apparently no development. 



Agar shmt. — Thin, translucent, slightly pinkish, shining growth; not widely spreading, 

 usually comjiosed of discrete and confluent colonies. 



Houillun. — L'inkish sediment and with strands more or less in suspension. The medium is not 

 clouded to any extent, and there is no inycoderm. 



Potato. — Elevated, pink or jiale reddish, dense, circumscribed layer, sharply defined as to its. 

 margins, with a rather rough surface. 



Litmus mill!. — No coagulation or change in color after two weeks. 



Sugar gelatin in deep stab. — No development. 



L'osoiic acid. — Little effect on the color. 



Tndol production. — Faint reaction. 



Relation to temperature. — Grows at the temperature of the room and at ^5° to 36° C. 



Bacillus flavocoriaceus (Adametz-Wicbmau).' 

 . Sulphur-yellow bacillus (Eiseuberg).^ 



Isolated from the water of the Schuylkill lliver. Observed but once. A small, short,. 

 nonmotile, bacillus, Avith rounded ends, occurring often in pairs, and showing a tendency to 

 gather in small clumps. 



No spore formation observed. 



Colonies in gelatin. — About the fifth or sixth day the colonies on the surface appear as pale, 

 yellow, rounded, shining, semitranslucent disks, 1 mm. to L* mm. in diameter. 



Under a low power they are finely granular and yellow in color, becoming thinner and color- 

 less at the margin, which is smooth and shari)ly defined. 



There is often a central nucleus of a brownish yellow or greenish yellow tint. The deep 

 colonies under a low i>ower are rounded, and have a smooth, sharply defined outline. They are 

 granular, and yellow in color, sometimes with a greenish tint, and are somewhat refracting. 



Gelatin slant. — Narrow, pale yellow, semitranslucent strii^e, with a finely scalloped or dentated 

 margin. 



Acid gelatin. — Growth is less vigorous. 



Agar slant. — Thin, pale, yellow, narrow stripe of discrete and confluent colonies. 



Bouillon. — Clouded. Yellowish sediment. 



Potato. — Scanty pale yellow, moist, rather thin growth. 



Litmus mill\ — No noteworthy eft'ect. 



Sugar gelatin in deep stab. — Very little growth, if any. 



' Mittheilungen d. Oest. Versuchstation, 1888, Heft II. Keferenoe given by Lustig: Diaguostica dei batterL 

 del le acque. 



- Bakteriologische Diagiiostik. 



