43 G MEMOIES OF THE JfATIO:N^AL ACADEMY OF SCIENCES. 



Acid geJafin. — Grows well. Does not seem to develop any yellow color. 



Af/ar sidiit. — Wliitisli or grayisli tiut, not widely spreading strijie, with finely dentated or 

 scalloped margins. The agar, after a time has a brownish green color. In sugar agar stab the 

 medium becomes a deep brown color near the surface. 



BoiiiUon. — Clouded. Some sediment. Xo pellicle. 



Potato. — Thin, viscid, dirty brownish, widely spreading layer. 



Litmus milk. — No coagulation. Milk is decolorized, and later acquires a brown color, which 

 gets deeper and of a chocolate color as the culture grows older. There is a brownish ring on the 

 wall of the tube, and above this a bluish red ring; the reaction is acid. 



Sugar gelatin in deep stab. — Fair growth; no gas production; at the surface of the medium 

 where there is good growth, a brown color is produced in the gelatin. This brown color is 

 apparently ilue to the presence of the glucose in the culture medium, for it has also been observed 

 in sugar agar, as noted above. 



liosoUc acid. — Color is deepened. 



Tndol iwoduction. — Good reaction; does not form nitrites. 



Relation to temi)erature. — Does not grow at 35° to 30° C. 



Bacillus latericeus (Eisenberg).' 

 Ziegelroter bacillus (Adametz).'- 



Isolated from the water of the Schuylkill Eiver. Rare. A medium-sized, elongated, non- 

 motile bacillus, with rounded ends, often somewhat curved and growing into short threads; no 

 spore formation observed. 



Colonies in gelatin. — After about a week the surface colonies are small, round, elevated, 

 shining, reddish in color, semitranslucent, and have a sharply defined outline. The diameter of 

 the largest is less than 1 mm. Under a low power they are refracting, of a warm, reddish brown 

 •or red tint, and have a rounded, smooth, sharply defined contour. The deep colonies are rounded 

 and granular. The colonies never attain any size, and they grow slowly. 



Gelatin slant. — Narrow, elevated, shining, viscid-looking stripe, of a brownish red to a dark 

 ■yermilion color. 



Acid gelatin. — Grows well. 



Agar slant. — Pale, reddhsh brown or yellowish brown, narrow, smooth, moist, shining and 

 semitranslucent stripe, with wavy, sharply defined margins. 



Bouillon. — Clear, some stringy sediment; the reaction is alkaline. 



i^info.— Thin, reddish, moist-looking, not very widely spreading growth. 



Litm us m ill: — Decolorized and coagulated. The clot forms a level floor for a layer of brownish 

 red colored, cloudy serum above, and on this floor a reddish sediment accumulates as well as at 

 the bottom of the tube. The layer of serum deepens very slowly. The reaction is neutral. 



Sugar gelatin in deep stab. — No growth. 



Bosolic acid. — Color somewhat deepened ; reaction is slightly alkaline ; growth is not vigorous. 



Tndol production. — Negative; does not grow vigorously. 



Melation to temperature. — Does not grow at 35° to 36° C. 



Bacillus fluoresceus incognitus. 



Isolated from the water of the Schuylkill River. A medium-sized, motile, rather short bacillus, 

 with rounded ends, occurring often in pairs and sometimes in threads. In fluid media grows in 

 long chains. It has a polar flagellum. No spore formation observed. 



Colonics in gelatin. — On the second or third day the surface colonies are thin, translucent 

 exi^ansions, with irregular, wavy, sharply defined outlines, and of a diameter of several millimeters. 

 Sometimes a central, whitish nodule may be seen. Under a low magnifying power the colony is 

 translucent and slightly granular, taking on a slight yellow-brown tint toward the center, where 



> Sternberg, Man. Baet., 1892. p. 628. 



- Adametz. Die Bacterieu iler Nutz- uiul Trinkwasser, Vienna, 1888. 



