MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 437 



tliere is often a small, rDiinded, sliarply dctined relVactiiig nuck'us of tin- same color. (See PL I, 

 fig. 3.) The outline is sbari)ly ilefmed, but very irreg'iilar and sinuous, and the surface of the 

 colony is marked by numerous delicate lines wliicli sometimes brancli, suggesting, in connection 

 with the sinuous outline, the appearance of a leaf with its veins. The deei) colonies are rounded 

 or oval and sliarjjly defined in outline. They have a yellowish brown tint, and are finely granular 

 and not dense. In a day or two the colonies have a greenish tint, and few ia number may attain 

 a diameter of (i mm. to 8 mm. The gelatin around them acquires a bluegrecn fiuorescence. 



Gelatin slant. — Thin, translucent, slightly greenish stripe, with wavy outlines, not spreading 

 very widely. The gelatin takes on a blue-green tluorescence and becomes cloudy at the surface. 



Acid gelatin. — Growth not so vigorous and less green color produced in medium. 



Af/nr ,<ilant. — Moist, thin translucent grayish stripe, not widely spreading, and with wavy 

 mai'gins; the agar acquires a green color. 



Bouillon. — Clouded; delicate grayish pellicle on surface and whitish sediment. After some 

 time a greenish color may appear. 



Potato. — Moist, viscid, spreading brown-colored growth. 



Litmus ntilh: — Growth is apparently very slow; after a month or so maybe somewhat decolor- 

 ized or ])iukish, with a pink ring on tube wall; no coagulation. 



Sufjar (jclatin in dee}) stah. — Fair growth; no gas production. 



liosolic acid. — I'rnbably decolorized. 



Indol production. — Faint or doubtful reaction; does not produce nitrite. 



Relation to tempcrattire. — Does not grow at 35° to 3G° C. 



Bacillus fluovescens foliaceus. 



Lsolated from the water of the Schuylkill Itiver. Observed several times. A medium-sized, 

 motile bacillus, of variable length, with rounded ends, occurring singly, in pairs, and in long, 

 slender forms. It has a polar flagellum. Xo spore formation observed. 



Colonies in gelatin. — About the tliird day the surface colonies are whitish, semitranslucent 

 expansions, -with irregular bulging outlines and a diameter of 2 mm. or 3 mm. Under a low power 

 they appear rather thick, and marked by irregular, heavy, brown contorted bands and stripes, 

 running in a radical direction from the center, where there is often a dark nucleus. (See PI. I, 

 fig. 4.) Between these markings the colony is clearer and of a warm brownish tint toward the 

 center. At the margin the colony is thinner, and the outline is irregular and somewhat wavy, 

 but sharply defined. After some days the colonies have becon^e thicker, denser, pale green in 

 color, and, if few in number, they may attain a diameter of a centimeter or more, while the gelatin 

 acquires a blue-green fluorescence far beyond their margins. The colonies have a lobate outline 

 and are marked by slight radiating furrows or ridges, so that they have the appearance of a broad, 

 rounded, pale green leaf. These appearances are best seen wheu the colonies are very few and 

 when the gelatin has been made with meat infusion. 



The deep colonies are rouuded, sharply defined in outline, brownish in color, and may be marked 

 by indistinct radiating lines. 



(fclatin slant. — A widely spreading, pale greenish, semitranslucent layer, with rather coarsely 

 lobed margins, with a denser line or .slight furrow coiTesponding to the line of inoculation, and 

 smaller lines or furrows running laterally to the margin of the layer, the whole suggesting the 

 appearance of a long, slender, pale green leaf; the gelatin acipiires a nuirked blue-green fluores- 

 cence; these appearances are not well seen on gelatin made with meat extract. 



Acid gelatin. — Good growth ; no fluorescence. 



Agar slant. — Grayish white, semitranslucent, rather elevated and shining, not very widely 

 spreading layer, with wavy margins. The agar acquires a green color. 



Bouillon. — Clouded with the formation of a thin, grayish pellicle and a flocculeut sediment. 

 The bouillon acquires a green color. 



Potato. — ^loist, viscid looking, brownish, rather thick and widely spreading layer. The 

 growth may have a slightly mottled appearance. 



