MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 439 



ill outline. The gelatin iicqnires a faint green color. This marked wrinkling of the growth doe.s 

 not seem to lie present when the gelatin is made with Liehig's meat extract instead of the meat 

 infusion, the growth in general being less vigorous and producing little or no green color in the 

 gelatin. 



Acid (ji'Jatin. — Good growth. 



Agar slant. — Semiti'anslucent, not widely spreading, grayish or greenish white stiii)e, with 

 irregular or scalloped margins; very delicate wrinkles are seen in the growth, which give it a 

 granular ap|)earanee. The agar ac(iuires a greenish color. On agar made with Liehig's extract 

 the growth is much less typi('al. 



Bouillon. — Clouded, with a whitish, thin, friable pellicle on the surface and a whitish 

 sediment. 



Potato. — Brown, moist, viscid looking, thick, and rather widely spreading. The growth has a 

 somewhat mottled apjiearance. 



Litmus milli. — Coagulated and pink in color above, with little or no serum and white below; 

 reaction is acid; eflect is slow. It would seem that at tirst there is a deejiening of the blue color 

 and an alkaline reaction; this is doubtlui. 



Siu/ur (jchttiii in deep stab. — No development. 



L'osolic acid. — Color seems to be somewhat deepened. 



I ndol production. — Faint reaction; does not produce nitrites. 



Relation to temperature. — Does not grow at 35° to 30° C. 



Bacillus ambiguus. 



Isolated from the water of the Schuylkill River. A small, motile bacillus, with rouiuled euds 

 occurring singly, in pairs, and in long forms. It has a terminal flagellnm. 



Colonies in rjclatin. — On the third or fourth day the surface colonies are grayisli. translucent, 

 shining, slightly elevated disks with slightly irregular, sharply defined outlines, and about 1* mm. 

 in diameter. Under a low magnifying power they are finely granular toward their centers, darker 

 and yellowish brown, where there is a small nucleus, but thin and translucent toward their 

 margins, which are slightly wavy and sharply defined. About the centers of the colonies some 

 coarser scattered granules or a few indistinct short lines may be seen. The deep colonies, under 

 a low power, are rouuded and sharply detined in outline, finely granular and brownish, becoming 

 darker toward their centers. To the nuaided eye they appear as small brownish spots, and are 

 quite visiltle against a white background. 



(idatin slant. — A narrow, translucent, grayish stripe, with sharply defined, finely scalloped, or 

 slightly irregular margins. 



Acid gelatin. — Grows well. 



Agar slant.— 'Na.vTOW, translucent, grayish stripe, with sharply defined, finely scalloped 

 margins. 



Bouillon. — Clouded, stringy sediment; no pellicle. 



Potato. — Thick, viscid looking, gray cream colored, widely spreading growth. 



Litmus milk.—Pmk above, white below; reddish ring on the tube wall; the milk is not 

 coagulated until after about a month or more, and may not be then: reaction is acid. 



JSugar gelatin in deep stab. — Fair growth; no gas jiroduction. 



liosolic acid. — -Color is not markedly afi'ected. 



ludol production. — Gives an easily recognized but not marked reaction; does not produce 

 nitrites. 



Relation to temperature. — Grows at the temperature of the room and at 35" to 36° C. 



Bacillus recuperatus. 



Isolated from the water of the Schuylkill River. A small, not actirehj motile, bacillus, with 

 rounded ends, occurring in short and long forms and sometimes in threads: often in pairs and 

 showing a tendency to form clumps which are motile. Flagella could not be demonstrated; no 

 •Spore formation obsei'ved. 



