MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



445 



■of ill-defined lateral outgrowths. The growth along the line of inoculation is I'eeble; li(iuefactiou 

 slowly extends to tiie tube wall and downward for a short distance, the bottom of the li(iuefaction 

 becoming a horizontal plane on which a yellow sediment accuniulatcs and farther develoiinient 

 ceases. The liquefied gelatin has an alkaline reaction. 



Acid (jelatin. — Grows feebly or not at all. 



Agar slant. — Translucent, yellow, shining, not widely spreading gmwtli, with wavy, not 

 sharply defined margins. 



Bouillon. — Clouded, yellowisli sediment and yellowish Halves on the tube wall at the le\el of 

 the liquid and sometimes on surface. 



Potato. — neep orange colored, thick, elevated, moist, and widely spreading, with a somewhat 

 uneven surface. 



Litmus Diilk. — -Slowly decolorized ; no coagulation after six weeks; a yellow scum fornis on the 

 surface, some yellow sediment and a brown-yellow ring on tube wall, with a Iduish ring above, 

 reaction neutral. 



SiKjar gelatin in deep stab. — No growth. 



Eosolic acid. — Decolorized; reaction alkaline. 



Indol production. — Keaction doubtful. 



Relation to temperature. — Grows at the temperature of the room: does ]U)t grow at .3.5'^ 



to 30° C. 



Bacillus piillulans. 



Isolated from the water of the Schuylkill River. Not a rare species. A small, short, motile 

 tacillus, with rounded ends, occurring often in pairs. It is provided with several flagella; no 

 i5i)ore formation observed. 



Colonies in gelatin. — About the second or third day the surface colonies are yellow, shining, 

 slightly elevated, rounded, semitranslucent disks, thinner and whiter at the margins, with 

 shari)ly defined outlines, and about 2 mm. in diameter. When the colonies first appear they are 

 whitish and become yellow later. Under a low jwwer they are yellow in color, finely 

 granular and rather dense, but becon'iiig thin and colorless at their margins, which 

 are smooth or slightly wavy and sharply defined. A characteristic thing is the 

 liresence of coarse, rounded, or sausage-shaped granules on the under surface of the 

 colony, sometimes scattered irregularly and sometimes collected at one or more 

 Ijoints. The deep colonies are dark, granular, rounded, or slightlj^ irregular in outline, 

 and sharply defined. Sometimes buds or ])laqnes develop on the surfaces of the deep 

 colonies, and in older in^eparations like collections of small daughter colonies may 

 "be formed. The surface colonies slowly become surrounded by zones of hazy, liquefied 

 gelatin, with well defined circular outlines. A peculiar aromatic odor is developed in 

 mature plates. 



Gelatin stab. — First a j-ellow, shining, slightly elevated, rounded patch forms at 

 the i>oiut of puncture, with a fair growth along the line of inoculation; later liquefac- 

 tion occui's in the form of a cup funnel or inverted cone constricted at the top, where 

 there is an air space, and all the gelatin is slowly liquefied. The liquefied gelatin 

 contains yellowish fiocculi in susjieusion, and there is a tlocculent j'ellow deposit in 

 the lower portions, while on the surface a yellow clump or scum may be seen (fig. 5). 



Acid gelatin. — Grows somewhat less vigorously with little or no liquefaction 

 along the line of inoculation; but the liquefaction extends to tube wall and downward with a 

 rounded flow; the liquefied gelatin has a slightly alkaline reaction. 



Agar slant. — A narrow, yellow, semitranslucent stripe with finely wa\y or finely scalloped 

 margins. 



Bouillon. — Clouded: grayish setliment at bottom; grayish flakes on the surface, which may 

 form an imperfect pellicle. 



Potato. — ^loist, viscid looking, brownish yellow, shining, widely sju'eading, rather thick 

 growth. 



Litmus milk. — Decolorized and yellow ring on tube wall, with a yellow scum on the surface; 

 coagulation also occurs, with some cloudy yellowish serum above; the reaction is uncertain. 



