4-18 MEMOIKS OF THE :NATI0NAL ACADEMY OF SCIENCES. 



Rosolic acid. — Decolorized slowly ; reaction is alkaline. 



Indol proilucHdH. — Faint reaction; does not produce nitrites. 



Relation to tei)H)eraturc, — Grows at temperature of the room and at 3.5° to 30° C. 



Bacillus hsematoides. 



Isolated from the water of the Schuylkill Eiver. Eare. A medium-sized, elongated, nonniotile 

 bacillus, with blunt ends. It shows irregularity in staining and in form. jS^o spore formation 

 observed. 



Colonies in gelatin. — After about Ave or six days the surface colonies are small, shining, 

 slightly elevated, semitrauslucent, vermilion-colored disks, with sharply defined, smooth, rounded 

 outlines. Under a low power they are homogeneous, refracting, and of a bright red color, and have 

 sharp contours. Deep colonies are rounded, sharply defined in outline, granular, and yellowish 

 red in tint. The gelatin is slightly liquefied after a long time. 



Gelatin .slant. — A bright red, rather rough, shining, and dense membranous stripe, with 

 sharply defined, somewhat irregular margins. It seems to be formed by the fusion of small 

 rounded colonies, and thus has a rough or granular surface. After some time the gelatin is 

 slowly liquefied beneath the growth. 



Acid (jdatin. — Grows well. 



A(jar slant. — Pink-colored, rough growth, composed of confluent, pink, shining, rounded, and 

 elevated colonies; does not spread widely. 



Potato. — Bright red, elevated, numeroiisly wrinkled or coarsely granular looking growth, 

 spreading fairly well. 



Litmus milk. — Not decolorized or coagulated. There is a rich red wing of the growth on the 

 tube wall and a red sediment at bottom, where there appears to be some decolorization. The 

 reaction is alkaline. 



Sugar gelatin in deep stab. — ^'ery slight development. No gas production. 



Rosolic acid. — Color deepened slowly. 



Indol production. — Negative. 



Relation to temperature. — Does not grow at 35° to 36° C. 



Bacillus fluorescens Schuylkilliensis. 



Isolated from the water of the Schuylkill Eiver. A rather small, short, motile bacillus, with 

 rounded ends, occurring often in pairs and sometimes in threads and long forms. It has a polar 

 tlagellum. No spore formation observed. 



Colonies in gelatin. — On the second day the surface colonies appear as grayish white, semi- 

 translucent disks, with smooth, sharply defined outlines, and a diameter of about li 

 mm. Under a low magnifying power the colony is seen to be brownish and granular 

 toward the center, but thin and translucent toward the periphery. (See PI. I, fig. 10.) 

 The outline is smooth or slightly wavy, and sharply defined. From the center of the 

 colony brownish, indistinct, wavy, broken lines are seen to radiate towanl the margin. 

 The deep colonies are rounded or oval, dark brownish and granular, with sharply defined 

 outlines. In these, also, faint brownish lines are seen radiating from the center. Later 

 tlie surface colonies get thicker and larger and may have a greenish white color, while 

 the surrounding gelatin acquires a blue-green fluorescence. The colonies gradually 

 sink into the gelatin and a slow liquefaction begins, the colony losing its sharply defined 

 outlines and being surrounded by a narrow zone of cloudy liquefied gelatin. 



Gelatin stall. — Slow liquefaction ; first an excavation in deep saucer form, several 



millimeters in diameter and lined by a gray-white, thick layer, which is fairly smooth 



^ and shining (fig. 0). This excavation increases somewhat in size and liquefaction slowly 



occurs beneath the lining membrane, while the nonliciuefied gelatin near the surface 



acquires a marked blue-green fluorescence. There is only a faint growth along the line of 



inoculation. 



Acid gelatin. — Good growth; less fluorescent. 



v_/ 



