MEMOIRS OF THE NATIONAL ACADEMY OF SCIENCES. 



455 



Lit lima milk. — Coagulated and decolorized; the clot is firm and the serum more or less clouded ; 

 there may be more; or less pink coloration in the clot; the reaction is neutral or slightly acid. 



Si(<i'ir iirldtiii in (lecp stall. — (iood growth, similar in appearance to the growth in plain gelatin; 

 CO gas ]>roduction. 



Ro,solic acid. — Decolorized; reactio]i alkaline^ 



Indol production. — Negative. 



Relation to tem2)cr(itiire.— Grows at the temperature of the room and at 3-")^ to 30^ C. 



Rrmark.'i. — This bacillus has been described by the Frauklauds (Zeitschrift fiir Hygiene, Bd. 

 VI, ISSi), p. 388) under t1ie name 15. ramosus. They state that they have freciuently met with it 

 in the waters of the Thames and the Lea, and consider it to be identical with the Wurtzel bacillus 

 of l';isenberg (IJacteriologische Diagnostik) and of C. Fraiikel ((irundriss der Bacterienkunde). 

 LTnder the latter name it is mentioned by Tils (Zeitschrift fiir Hygiene, Bd. IX, p. 291, 1890) as 

 being one of the more common organisms in the Freiburg water supply. Pokrowsky found it in 

 the water of the Kura lliver. (Centralblatt fiir Bact. u. Par.. Bd. X, 1891, p. '>C>(j.) 



Bacillus siiblanatus. 



Isolated from the water of the Schuly kill Piver. A medium-sized, motile bacillus, with rounded 

 ends, occurring sometimes in pairs and in long forms; it has several tlagella; Ibrms 

 rounded, spores situated in swellings near the ends of the rods. (See fig. 17.) 



Colonir.s ill (jclatin. — About tlie second day the surface colonies appear as rounded 

 grayish disks, 1 mm. to 2 mm. in diameter. LTnder a low power they are granular, 

 rather dense toward their centers, but become more translucent at their margins, which 

 are sharply defined and nearly smooth. The deep colonies are generally round, brownish Fr.i. n. 

 and granular, not dense, and sharply contoured; under some circumstances they may 

 forin colonies or aggregations of small daughter colonies. The original colony in the " water" plate 

 was a radiating figure formed of columns of these daughter colonies; but no such characteristic 

 appearance has beeu seen in the colonies derived from this. About the third day liquefaction 

 is present, and the surface colonies have formed round, saucer-shaped li(|nefactions, 

 which are yellowish white at their centers, while in the cloudy, liquefied gelatin which 

 they contain a hazy ring may be seen. Under a low [>ower these liquefied areas ai-e 

 dense aiul granular, toward their centers brownish, while at their margins a fringe of 

 radiating fibrils maybe seen. 



Gelatin stab. — Liquefaction in deep saucer form constricted at the top (fig. 18). This 

 gradually extends to the wall of the tube and also downward with a rounded fioor, 

 eventually becoming level. There is faint growth along tlie line of inoculation; the 

 liquefied gelatin is clouded with a whitish mycodei-m at the sui'face. and after a tune 

 an abundant, whitish sediment; an alkaline reaction is produced. 



^•lr/(/ (jilatin. — (Irowth is perhaps less vigorous and the liquefied gelatin acquires 

 a neutral reaction. 



Aijar slant. — Translucent, thin, grayish, narrow stripe, with sharjily defined 

 margins: the growth seems to thrive better beneath the surface of the agar: the 

 V J nu'dium acquires a brownish green color. 



Tio «//?(>». —Clouded with a whitish sediment; the bouillon acquires a greenish tint. 

 Potato. — Brownish, thin, granular looking, rather widely spreading moist growth. 

 Litm IIS milk. — No coagulation ; the milk is decolorized and the casein seems to go into solution ; 

 there is a bluish ring in the tube wall and the reaction is alkaline. 



Siiffar gelatin in deep stab. — Only very faint development after some weeks; no gas. 

 L'osolic acid. — Apparently no growth. 



Jndol production. — Does not grow vigorously: reaction faint or doubtful: the addition of 

 nitrite solution is necessary. 



Relation to temperature. — Crows at the temperature of the room and at 35^ to 36° C 



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