276 



M. TSÜKAMOTO. 



Action upon Algae. 



In 0.1 'Yo i^olntiori of:ilJvlic nlcoho] Spirihjtjnt (uvimiunis was killed 

 in 24 hours, while in the solutions of the other alcohols of the same 

 dilution it was found healthv even after 10 days. Tn other experiments, 

 allylic aleohol of 0.05 •'/„ also killed Spirofii/ni in 24 hours. Allylic 

 alcohol of O.OP/o killed it in three days, hut O.()05°/o had no effect even 

 in 10 days. In O.ö"/,, solutions, this alo^a was killed hy amylic alcohol 

 in one day, hy hut\lir in o days, hy isohufylic in 4 days, still later hy 

 tertiarv hutvlic, ju'opvlic, and isopropvlic alcohols. In dilutions oi' 

 1 °/o. methvli<* and ellivli«' alcohols had no injurious effect, hut 

 ])ropylic alcohol killed the cells wifhin ,") days, and isopropylir, hul-ylic, 

 isobutvlic, and tertiarv hutylic alcohols killed it witliin '2 days.' In 

 '2^1^ solution, ethylic alcohol proved nuich more irijurious than metli- 

 ylic, for while most of the cells were dead aüei- H days in the former, 

 only a few were so in the latter, l)ut in either alcoliol all the cells 

 were dead after 5 (Liys. In a o"/o solution of ethylic alcohol all were 

 killed within o davs, while in that of methylic alcohol tliey lived 

 4 davs. In a 4"/o solution of methylic alcohol, however, all the cells 

 were killed within two days. 



Action upon Microbes. 



For these experiments principally methylic, ethylic, ainylic, and 

 allylic alcohols were used. In one series of experiments their toxical 

 actions were tested and in other series their nutritive qualities. On(^ 

 drop of putrefying- hroth was introduced into the alcoholic solution of 

 a certain dilution, and then a sterilised solution of meat-extract \vas 

 infected from the alcoholic solution after this had stood 24 hours.- 

 The results ai"e shown in tlie folIowinL^- tahles : 



1. [u auother case r;o isopropylie alcohol solution killed Spiruyi/ni cells ^rithin one clay. 

 :i. Of course, control experiments with plain water were also made. 



