278 



M. TSUKAMOTO. 



: nfter 4 days, considerable development. 

 : after 14 days, large development. 

 : after 14 daj's, quite clear, free from 

 bacteria. 



no development, 

 stronii" development, 

 weak development, 

 no development. 



In a third series ol" experiments no source of carbon for the 

 growth of bacteria was contained in th(^ solution except the alcohol 

 itself, the other constituents being only 0.5 'Yo ^h'^^t^ 0.1 ^/o potassium 

 phosphate, and 0.01 "/o magnesium sulphate. The infection was made 

 from putrid meat and the flask left to stand at the ordinary tempera- 

 ture. The results were as follows : — 



O.P/o îdlylic alcohol : after 9 days, slightly turbid ; a few 



bacteria. 

 : after 24 days, quite clear, no bacteria. 

 : after 14 days, no bacteria. 

 : after 10 days, slight tui-bidity ; small 

 oval ijeaü cclh^ were seen but no bacteria. 

 : after 5 days, slight turbidity ; small oval 

 shaped bacteria only were seen.- 

 In a further series of experiments I compared the nutritive effect 

 of the nine alcohols in dilutions of O.P/q with only the addition of 

 0.5*^/0 ammonium phosphate, 0.1*^/,) monopotassium ])hosphate, and 

 O.Ol^/o magnesium sulphate. These solutions were all infected from 

 the same source, viz : a Pasteur's solution that had been exposed in 



0.5% ., 



P/o amylir 

 10^0 ethylic 



10% methylic 



1. Compare O Lœw, Natiirl. System der Giftuirliiingcn, S. 20. 



2. According to li. Brown (Chem. Soc. Jour., 1886.) Bacterium aceti utilises uiethylic but 

 not amylic alcohol. 



