STUDIES OF REPRODUCTIVE ELEMENTS: II. 3()3 



as Figs. 35 or 36, the number of chromosomes counts greatly above 

 this, and I am not, at present, able to tell exactly their num- 

 ber. 



2. Fropluisis : — Figs. 31, 33, and 34 are taken from preparations 

 of nuclei which are just preparing to divide. In Fig. 31 the chromo- 

 somes are not very distinctly to be seen, owing to the deep staining 

 with hematoxylin. We see, however, on the left hand side a 

 curved double line undoubtedly representing the segmentation of one 

 of the chromosomes. Tliis is very beautifully seen in preparations 

 represented by Figs. 33 and 34, both of which are stained with a 

 combination of acid-fuchsin and methylen-blue. In hoth. nuclei the 

 chromosomes are seen to be composed of a double row of minute 

 microsomes, the number of which in a single chromosome is not 

 with certainty to be determined, since the entire length of a chromo- 

 some is very rarely to be made out. In a few cases, however, I count- 

 ed them to be ten or twelve in a single row, as wdll be seen in the V- 

 shaped chromosome, lying a little to the left, in the nucleus represented 

 by Fig. 33. A nearly equal number will be counted, though not so 

 clearly, in one of the two chromosomes at the right hand of the same 

 nucleus. Similar states of chromosomes are seen in Figs. 44-1:7, 

 drawn from preparations of s])ore-forming individuals in about the 

 same stage as that represented by Fig. 18. In Fig. 44 the chromo- 

 somes, except a few, do not seem to lie in any definite order, but to be 

 scattered more or less irreo-ularlv in the nucleus: but in Fig-s. 33, 34 and 

 45, they lie more or less parallel with each other, their long axis being in 

 the direction of the archoplasm found just external to the nucleus. 

 In Fig. 46 the above position of the chromosomes is not so clearly 

 expressed as in the figures just mentioned, although the archoplasm can 

 be distinctly seen on one side of the nucleus. This, however, is only 

 an optical illusion caused by the position of the archoplasm which does 



