308 



C. ISHIKAWA. 



ject is looked at a little from one side, the central filtres (c. f.) 

 lying in the furrow of the nucleus, and the radiating fibres (r. /.) 

 proceeding from tlie upper archoplasm, are distinctly visible. It 

 thus seems that segment ati(^n of the chromosomes in spore-formation 

 takes place before the previous division of the nucleus is yet at its 

 end. This is also to be looked upon certainh^ as the result of the 

 rapid progress of the bud-formation. 



4. Anaphasis : — The details of anaphasis can only be observed 

 in individuals which divide, since in those which foru) sjjores the 

 nucleus divides successively without the intervention of any resting 

 stages, as mentioned above; and at the end of the spore-formation the 

 diminished size of the nucleus makes it very ditficult to observe the 

 individual chromosomes with any accuracy. I will therefore only 

 give my obser\ations of the changes, at this stage, in the nuclein 

 subs<tance of the dividing individuals. Fig. 38 represents one of tiie 

 nuclei soon after division. The parallel arrangement of the chromo- 

 somes, their relative position in regard to tlie archoplasm («), and the 

 geijeral shape of the nucleus are all dis[)layed very pklinly. The 

 positions of some of the chromosomes at its right lower corner, how- 

 ever, become more or less irregular in their relations. Fig. 40, which 

 represents one of the nuclei nearly at the stage of Fig. 7, shows that 

 the chromosomes have lost their parallel arrangement. The micro- 

 somes are, however, still very small, represented only ])y small dark- 

 coloured dots. In Figs. 39-43 the microsomes have become somewhat 

 swollen up, assuming nearly the characters of those in the resting 

 nuclei. The microsomes in Fig. 42, however, appear to be a little too 

 large, owing perhaps to the action of the acetic acid with which the 

 specimen was treated. 



5. The fate of the arcliuplasin : — The archoplasm, as we have 

 stated, comes to be seen first at the stage a little before the division or the 



