332 



C. ISHIKAWA. 



stage of division to tbat represeiited on the left of the figure). Acetic acid methyl- 

 green preparation. 



Fig. 19. Stage similar to that of fig. 18, sho-\ving line of partial division 

 of animal. Picro-acetic acid preparation without staining. 



Plate XIII. 



(Figs. 20-30, represent spore-formation; Figs. 31-37, dichion.) 



Fig. 20. Stage of sporulation more advanced than that of fig. 19, showing 

 also line of partial division of animal. (It will he observed that the number of the 

 nuclei in each half is not equal, four lying on one side and two on the other). 

 Acetic acid methyl-green preparation. 



Fig. 21. Stage of spore-formation still later than above, showing also line of 

 partial division, six nuclei lying on one side and four on the other. Acetic acid 

 methyl-green preparation. 



Fig. 22. Stage with fifty-one nuclei seen laterally ; spore-cells on the other 

 side represented by fainter shading. Acetic acid methyl-green preparation. 



Figs. 23-2G. Sections of spore-buds in last stage of division. Killed with 

 Flemming's stronger fluid and stained with combination of Bohmer's hfematoxylhi 

 and rubin. 



Figs. 27-30. Eipe spores just before detachment from the mother-body. 

 Killed with Flemming's stronger solution and stained with carbolic acid fuchsin. 



Fig. 31. Part of an individual just preparing to divide ; very large archo- 

 plasm close to nucleus ; tentacle and teeth still visible. Killed with Flemming's 

 stronger solution and stained with Bohmer's hematoxylin. 



Fig. 32. Nucleus of individual preparing to divide. Killed with Boveri's 

 picro-acetic acid, and stained with acid-fuchsin and methylen-blue. 



Figs. 33 & 34. Nuclei of two dividing individuals, in which the longitudinal 

 division of chromosomes are beautifully to be seen. Killed with Flemming's stronger 

 fluid, and stained with acid-fnchsin and methylen-blue. 



Figs. 35 & 35. A. Nucleus in process of division as seen at two diflerent 

 levels: fig. 35 at level of archoplasmic spindle, and fig. 35. A. at a lower level ; 

 centrosome within the archoplasm on the lower side. Killed with F]( inming's strong- 

 er fluid and stained with Bohmer's hfematoxylin. 



