AND THE FOETAL MEMBRANES, TN THE OHTOK-. '^^J 



e). Thei'e. tîikes plficc no ])eri"or:ilioii of the .scro-amniotic con- 

 nectioM in Clielonia. 



f). The sero-amniotic coiiiiectioii is quite witlioiit relation to 

 the «^uieroeiice of the yoniio- in the chick. 



g). Tliere is produced no alhumen-sac in (-helouia and therefore 

 the sero-amniotic connection is not enclosed within the foetal mem- 

 branes. Consequently the allantois of Chelonia is very dilterentlv 

 affected by the sero-amniotic connection. 



77, Methods for the Preparation of Spechiiens. 



From the second week of incubation it becomes ahiiost hoj)eles.s \o 

 try to take out the contents of an e^^^ entire in the fresh condition, 

 and the whole eg'g- con tents wliould tlierel()i'e be hardened, in the fol- 

 lowing manner. 



When ail incubated ^gg is looked at by transmitted light it should, 

 to be a promising one, be semi -opaque after the first week and entirely 

 so after the first ten days. Having obtained such an egg it siiould 

 be patiently tapped with a glass rod or something of the sort, until 

 the greater part of the shell is broken into small pieces, the shell then 

 taken off, piece by piece, leaving tlie underlying shell-membranes 

 intact. From this point the proeeduiv will be somewhat different 

 according as the egg has been incubated more than two weeks <m- less. 



a). lllieit tjie e<fif luis been in inciihation less than tiro weehs. 

 Care should l)e taken not to injure large blood vessels of the allantois, 

 which is closely attached to the inner shell-menil)]'ane especially near 

 the large pole: whenever they are injured by accident, the blood 

 may be cooled by bhjwing at the point, when coagulation soon sets in. 

 The shell is taken off in this way over an area about one inch square, 

 and an opening made into the air chamber, the position of which is 

 easily found out by the sound on tapping. 



