81 



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244. SHEAR, M. J. and ANDERVONT, H. B. 



Chemical treatment of tumors. III, Separation of hemorrhaee- 

 produclng fraction of B. coll filtrate 



Proc. Soc. Exper. Blol„ Med. 34:323-325, I936 



A method Is described for the separation of the tumor-hemorr- 

 hage producing fraction of B. coll filtrate from toxic and Inert 

 contaminants. The method Involves centrlf ugatlon, filtration, 

 alkaline precipitation, and the removal of calcium by acidi- 

 fication with HCl and precipitation with alcohol. The final 

 product was a water-soluble fraction, highly potent for the 

 production of mouse tumor hemorrhage In amounts as low as 0.1 cc, 

 of a 1;100 dilution of a stock solution containing 22 mg. per 

 cc. of the active fraction. 



245. SHWARTZMAN, G. 



Reactivity of malignant neoplasms to bacterial filtrates. II, 

 Relation of mortality to hemorrhagic necrosis and regression 

 elicited by certain bacterial filtrates 



Arch, Path. 21:509-524, I936 



Mice bearing 12-day-old sarcoma I80 implants were injected intra- 

 venously, intra-oeritoneally or subcutaneously with one of the 

 following bacterial filtrates: meningococcus "agar washlngs'j 

 B. enteritidis . B. coll , E, typhosa , Staphylococcus aureus . 

 Streptococcus hemolytic us and the tubercle bacillus. The ability 

 of the preparations to elicit the Shwartzman phenomenon in 

 rabbits was determined shortly before parenteral injection. 



E. typhosa and meningococcal filtrates were more toxic for the 

 tumor-bearing mouse than for normal animals; filtrates of B, 

 enteritidis were toxic for both normal and tumor-bearing test 

 animals. All three were potent agents for tumor destruction. 

 Bo coll , Str, hemolytlcus and tubercle bacillus filtrates pro- 

 duced only slight changes in tumor tissue. Staphylococcus aureus 



filtrate produced no changes. Since all filtrates were highly 



toxic, it was evident that the lethal effects and tumor-des- 

 tructive activity exist Independently, In certain given 

 proportions, serum-E, typhosa mixtures protected animals against 

 the lethal effects, while tumor hemorrhage, necrosis and re- 

 gression proceeded unchanged. 



