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249. BOYLAro, E. and BOYLAND, M. E. 



Studies In tissue metabolism. IX. The action of colchicine and 

 B. typhosus extract 



Blochem. J, 31:454-460, 1937 



Agar washings of E. typhosa cultures were injected to deter- 

 mine the effect, of tumor hemorrhage produced by such means upon 

 tumor, liver and intestine ascorbic acid content. Colchicine 

 was Injected and its toxicity for normal and tumor-bearing mice, 

 capacity for hemorrhage production in the transplanted tumor or 

 the mouse and rat and tissue metabolic changes were determined, 

 in vitro and in vivo. The effect of colchicine upon the as- 

 corblc acid content of tumor, liver and intestine and its ability 

 to produce the Shwartzman phenomenon in the rabbit were observed. 



Both colchicine and E. typhosa agar washings were capable of 

 producing tumor hemorrhage and reducing ascorbic acid content 

 and metabolism in grafted tumors, but dosages necessary to ini- 

 tiate these effects were close to the toxic levels. Tumor- 

 bearing animals were more sensitive to the toxic effects of 

 colchicine than normal animals. The size of the tumor and the 

 survival rate were significantly related. Ascorbic acid content 

 of the rat and mouse intestine diminished after colchicine; 

 liver metabolism was not inhibited. Colchicine did not elicit 

 the Shwartzman phenomenon, nor did it produce hemorrhage in the 

 liver, 



250. CO TUI, McCLOSKEY, K. L., SCHRIPT, M. and YATES, A. L, 



A new method of preparing nonpyrogenic Intravenous infusion 

 fluids, based on removal of pyrogen by absorptive filtration 



Ann, Surg. 106:1089-1094, 1937 



Adsorptlve filtration, followed by ultrafiltration, is advocated 

 for the preparation of pyrogen-free Intravenous solutions. The 

 sterile solution is passed through the compressed Seltz EK 

 (serum) type asbestos filter and the 200-second Zsigmondy mem- 

 brane filter. The Jena, Berkefeld, and 42-second Zsigmondy 

 type filters are not effective for this purpose. It is be- 

 lieved that the pyrogen particle diameter lie between 50 milli- 

 micron and 1 micron. 



