93 



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 277. CO TUI and SCHRIFT, M, H„ 



Production of pyrogen In sera by bacteria 

 Proc. Soc, Exper. Biol. & Med. 42:5^9-552, 1939 



Normal horse serum was inoculated with cultures of Pr, vulgaris, 

 B. coli . Staphylococcus aureus , B. su btilis and two unidentified 

 organisms isolated from water. Following Incubation periods of 

 varying duration, the culture fluids were filtered and injected 

 intravenously in the dog, A pyrogenic symptom-complex which 

 included fever, leucopenia, occasionally chills, and gastrointes- 

 tinal disturbances, was noted, Pr, vulgaris, B. coli and Staphy - 

 lococcus aureus filtrates produced severe reactions. Pyrexia was 

 insufficient after B. subti lis injections, but leucopenia was 

 present, "On the basis of these experiments it is suggested that 

 the chill and fever-producing substances in sera may be pyrogens, 

 caused either by direct bacterial growth, or by pyrogen already 

 present in chemicals used in the purification and concentration 

 of therapeutic sera." 



278, no TUI, SCHRIPT, M. H, and RUGGIERO, W. F, 



Production of pyrogen in gum acacia by bacteria 



Proc. Soc. Exper. Biol, & Med. 41:533-535, 1939 



Pyrogen-producing bacteria (B. subtili s, two unidentified water 

 organisms and Staphylococcus "alFus ) grown In gum acacia solutions 

 produce an "active" a"cacla capabre of eliciting febrile responses 

 after injection. Leucopenia and other changes seen with reactive 

 inulin and typhoid vaccine follow. 



279^ DENNIS, Eo W. and SENEKJIAN, H. 



A leucocidal toxin extracted from typhoid bacilli 



Am. J. Hygiene 30:103-11, 1939 



A crude extract of typhoid bacilli presents two active factors: 

 a leukocidal factor with selective lethal effect on the pseudo- 

 eosinophilic granulocytes and a toxic somatic antigen. The 

 former loses activity on purification by trichloracetic acid, but 

 the latter increases in potency during the same process. Leuko- 

 cidin can be demonstrated in extracts of S. typhimurium, S, 

 schottmuleri,^ S, sulpestifer (voldagsen strain), S. enterTtidis , 

 S. gallin arum and S. paratyphi, listed in decreasing order of 

 activity. 



