98 



- 1 9 3 9 - 



J. Immunol. 375 507-524, 1939 



After a brief review of the use of bacterial products In the 

 therapy of malignant neoplasms and of methods for their prepara- 

 tion, details of a comprehensive study of the fractions obtained 

 from Salmonella typhimurium a re appended. 



Crude fractions, nucleoproteins, nucleic acid and p61ysaccharldes 

 were tested ^s preparatory or provocative agents incthe Shwartz- 

 raan reaction; all were found suitable for either role. Several 

 strains of transplanted sarcoma or carcinoma were allowed to 

 multiply for 10 to 21 days in the rat; intra-abdominal injections 

 of the bacterial fractions were then administered in 1 to 6 doses 

 at the tolerated levels. There were intervals between doses 

 which ranged from 3 to 7 days. Tumor hemorrhage, necrosis and 

 regression were seen in Sarcoma l8o on injection of taucleoprotelna 

 polysaccharides or fractions containing one or both of these 

 constituents. In a maximum percentage of rats sublethal doses 

 of these active fractions produced total regression. Pew car- 

 cioma strains underwent complete regression without recurrence. 

 The authors do not find a single special bacterial substance 

 responsible i'or activity in the Shwartzroan phenomenon and against 

 malignant neoplasms, but find that the ordinary proteins and 

 carbohydrates of the bacterial cell carry the hemorrhagic -ne- 

 crotic functions. 



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