-19^0- 



Antigenlc fractions from B» typhlmurlum and Bo typhosum have 

 been prepared by 3 methods j {sT"^ development of the tryptlc 

 digestion method of Raistrlck and Topley, (b) Bolvin's method 

 of extraction with cold trichloracetic acid, and (c) extraction 

 with organic solvents such as ethyleneglycol and diethyleneglycol 

 Morgan f 1937") and the products have been compared by chemical 

 analysis and determination of toxicity in mice. The purest pro- 

 ducts obtained by these methods show a similarity of properties. 

 It may be concluded that, by any of these methods, the essential 

 antigenic complex of the bacteria may be obtained in a state 

 approaching chemical homogeneity o 



On the grounds of yield and suitability for use in extracting 

 large quantities of bacterial bodies, the tryptlc digestion 

 method and Bolvin's method are to be recommended. 



302, GRAY, J. S.J, CULMER, C. U., WIECZOROWSKI, E. and ADKISON, J. L;' 

 Preparation of pyrogen-free urogastrone 



Proco Soco Exper. Biol, and Med. 43:225-228, 19^0 



Extracts of normal human male urine were prepared by 3 methods: 

 the benzoic acid adsorption procedure of Katzman and Dolsy; 

 further purification procedures Involving neutral acetone fraction- 

 ation. Assay of the gastric inhibitory factor was performed in 

 6 Heldenhaln pouch dogs in,jected subcutaneously with histamine; 

 rectal temperatures and percentage inhibition of free acid secre- 

 tion were recorded after injection of the extract. Pyrogen assays 

 were also made by recording rectal temperature curves for a group 

 of 5 rabbits. 



The pyrogenlc effect of the extracts was progressively lost with 

 the use of the more purified materials, though gastric inhibition 

 was not materially affected. Utilizing the acid acetone fraction- 

 ation procedure, the gastric inhibitory factor was obtained free 

 from pyrogen. 



"The results indicate quite clearly that the gastric inhibitory 

 factor is separate and distinct from the pyrogenlc factor in 

 extracts of normal male urine. Until such time as the gastric 

 Inhibitory factor is definitely identified, we propose to call it 

 urogastrone." 



303. GRAY, J. S., WIECZOROWSKI, E., CULMER, C. U. and ADKISON, J. L. 



The presence of pyrogen in urine and its separation from urogastrone, 

 Amer. J. Physiol., 1292589-596, 19^0 



