103 



_ 1 9 4 - 



This study was undertaken to determine whether the gastric in- 

 hibition produced by urine extracts was due to pyrogenic sub- 

 stances. Six Heidenhain pouch dogs were used for assay of the 

 gastric inhibitory factor under controlled conditions; the 

 potency of the injected material was measured by percentage 

 decrease in mgm, of free acid secreted. Pyrogen was assayed by 

 rise in rectal temperature of the pouch dogs and in rabbits. Crude 

 urine extracts were prepared by several modifications of the 

 benzoic acid adsorption method of Katzman and Doisy (methods 

 given) . Purification of the crude extracts was accomplished 

 through fractionation with acetone. 



It was found that the major portion of the pyrogen in the crude 

 extracts originated in bacterial contamination; measures which 

 minimize bacterial growth permit preparation of crude extracts 

 containing no more pyrogen than equivalent amounts of fresh urine o 

 Fresh, untreated urine from healthy human subjects xvas found to 

 contain small and variable amounts of pyrogenic substance » Purifi- 

 cation of crude extracts relatively free of pyrogen consistently 

 yielded products exhibiting high urogastrone and little or no 

 pyrogenio potency c 



It was concluded that urogastrone is not pyrogen. In disease 

 the quantity of pyrogen in the urine is increased. 



304. LEES, J. C. and LEWY, G. A. 



Emergency preparation of pyrogen-free water 

 Brit. Med. J., 1;430, 19^0 



A method is described for removing pyrogens from contaminated 

 water, thus rendering it suitable for intravenous use. The method 

 consists of shaking the water with powdered charcoal (1 part 

 per 1,000) for fifteen m.inutes, then allowing to stand for several 

 minutes after vjhich the charcoal is readily removed by decanting 

 through a filter paper. 



305. MORGAN, H, R. 



Preparation of antigenic material inducing leucopenia from Eber - 

 thella typhosa cultured in a synthetic medium 



Proc. Soc, Exp, Biol. Med. 43:529-532, 1940 



Small amounts of antigenic substance isolated from cultures of E. 

 typhosa induce fever and leukopenia in the rabbit while larger 

 amounts are lethal to both mice and rabbits. Leukopen.ia was the 

 result of the disappearance of neutrophils from the blood. The 

 toxic properties of the antigen remain after 30 minutes boiling. 



