105 



-19^0- 



Both substances, obtained In yields of 10 to 15 per cent of the 

 weight of dried typhoid organismsj give negative or faint 

 biuret reactions and a strong Molisch reaction o Nitrogen content, 

 corrected for ash and moisture, is from 3.^ to 4.6 per cent. 

 Hexosamine, if present, accounts for less than 10 per cent of 

 total N. Both substances give stroiig precipitin reactions with 

 antityphoid rabbit serum, 



PARTRIDGE,' S. M. and MORGAN, W. T. J. 



Immunization experiments with artificial complexes formed from 

 substances isolated from the antigen of Bact . shigae 



Brit. J. Exp. Path, 21:l80-195> 19^0 



A specific polysaccharide component of the antigen of B, shigae 

 failed to induce iiftmune bodies in the rabbit. The polypeptide 

 component of the same complex produced low titer homologous 

 precipitins but no agglutinins, A synthetic 'complex' built from 

 these components in combination induced the formation of potent 

 immune bodies, agglutinated B. shigae at high titer and precipi- 

 tated with the specific polysaccharide. The complex fixed comple- 

 ment when Shiga antibacterial sera was added „ 



SHAPIRO, C. J. 



The effect of toxic carbohydrate complex from S. enterltides on 

 transplantable rat tumors in tissue culture 



Am. J. Hygiene, 31:SeG, B. 114-26, 19^0 



Tissue cultures of rat sarcoma #10 or Walker carcinoma #256 were 

 maintained in vivo in healthy rats as intradermal, subcutaneous 

 or intramuscular growths. On plasma growth medium they were ex- 

 posed to a toxic carbohydrate complex derived from S. enterltides , 

 capable of causing tumor hemorrhage and necrosis in doses as small 

 as 0.0005 mg. Control tissue tumor cultures were maintaine'd in 

 the growth medium and saline. Doses of 2000 to 50OO provocative 

 units of polysaccharide were used in all in vivo experiments. No 

 effects were noted in vitro . It is believed that the plasma of 

 a tumor-bearing host does not interact with the hemorrhage -pro- 

 ducing agent to form a substance toxic to either young or sensi- 

 tive cells of these neoplastic growths, 



SHEAR, M. J. and TURNER, F. C. 



Studies on the chemical treatment of tumors. V. Separation of 

 the hemorrhage-producing fraction from. Bacillus prodlgiosus 

 filtrates 



