-19^0- 



J. Biol. Chetn„, 133:Lxxxvil, 19^0 (Abstract) 



Methods which had been used to separate hemorrhage -producing 

 fractions from Bo coli were applied to broth cultures of B. 

 prodiglosus . An untreated broth culture filtrate contained 330 

 mouse tumor units per cc of active principle. In a commercial 

 sample of Coley's mixed toxins, 100 units per cc, were present. 

 Further treatment of the above filtrate gave a concentration which 

 contained 20,000 mouse units per cc. In studying growth on simple 

 synthetic media, it was noted that filtrates had the same potent 

 quality as those from broth cultures. On treatment with CHCI3, 

 an emulsion was obtained which possessed most of the activity of 

 the filtrate, A water-soluble precipitate remained after CHCI3 

 was removed and C2H5OH employed, A solution of this concentrate 

 had a potency of 133*000 mouse units per cc „ and contained 0.2 

 gamma per mouse tumor unit. Lethal dosages of these concentrates 

 ranged from 100 to 1000 times the minimum effective dose. The 

 active principle was nondialyzable through cellophane and was 

 heat-stable. Large scale preparation techniques are given in 

 detail, 



SUTHERLAND, A, M, and WOLPP, H, G„ 



Experimental studies on headache; Further analysis of mechanism 

 of headache in migraine, hypertension and fever 



Arch, Neurol, and Psychiat, 44^929-949, 1940 



In twelve patients with chorea or rheumatoid arthritis, fever 

 therapy was induced by intravenous typhoid vaccine, A tambour 

 was placed on the temporal artery and the pulsations recorded. 

 Photographic records of the pulsations of the cerebrospinal 

 fluid were made from a needle introduced into the lumbar sac and 

 connected to a Prank capsule. Control records of temporal artery 

 and cerebrospinal fluid pulsations were made simultaneously. 

 Then an appropriate dose of typhoid vaccine was given intravenously; 

 and estimates of the degree of headache, determinations of blood 

 pressure, and pulsations of temporal artery and cerebrospinal 

 fluid were made at frequent intervals. 



Increased amplitude of pulsations of temporal artery and cerebro- 

 spinal fluid in all instances was found to be followed by onset 

 of headache or sensation of head fullness. Exacerbations and re- 

 missions of the headache were found to closely parallel the in- 

 crease or decrease in amplitude of pulsations. The headache 

 ended with the ultimate decrease in amplitude. These findings 

 are similar to those seen in histamine headache. Increasing the 

 intracranial pressure decreased the intensity of the headache. 



